ArticleJournal of virology2004
Strand-specific RNA synthesis determinants in the RNA-dependent RNA polymerase of poliovirus.
Article in Journal of virology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
10 citing papers in PubMed, 16 citations in OpenAlex.
- Article
- Comparative Molecular Biology Approaches for the Production of Poliovirus Virus-Like Particles UsingmSphere · 2020Article
- Involvement of a Nonstructural Protein in Poliovirus Capsid Assembly.Journal of virology · 2019Article
- An upstream protein-coding region in enteroviruses modulates virus infection in gut epithelial cells.Nature microbiology · 2019Article
- Increasing Type 1 Poliovirus Capsid Stability by Thermal Selection.Journal of virology · 2017Article
- Different domains of the RNA polymerase of infectious bursal disease virus contribute to virulence.PloS one · 2012Article
- Cellular protein modification by poliovirus: the two faces of poly(rC)-binding protein.Journal of virology · 2007Article
- Identification of determinants involved in initiation of hepatitis C virus RNA synthesis by using intergenotypic replicase chimeras.Journal of virology · 2007Article
- Allosteric effects of ligands and mutations on poliovirus RNA-dependent RNA polymerase.Journal of virology · 2005Article
- Differential rescue of poliovirus RNA replication functions by genetically modified RNA polymerase precursors.Journal of virology · 2004Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The viral RNA-dependent RNA polymerase (3D(pol)) is highly conserved between the closely related enteroviruses poliovirus type 1 (PV1) and coxsackievirus B3 (CVB3). In this study, we generated PV1/CVB3 chimeric polymerase sequences in the context of full-length poliovirus transcripts to determine the role of different subdomains within the RNA-dependent RNA polymerase of PV1 that are required for functions critical for RNA replication in vitro and in cell culture. The substitution of CVB3 sequences in the carboxy-terminal portion (thumb subdomain) of the polymerase resulted in transcripts incapable of RNA replication. In contrast, three of the seven chimeras were capable of synthesizing RNA, albeit to reduced levels compared to that of wild-type PV1 RNA. Interestingly, one of the replication-competent chimeras (CPP) displayed an inability to generate positive strands, indicating the presence of amino-terminal sequences within the 3D polymerase and/or the 3D domain of the 3CD precursor polypeptide that are necessary for the assembly of strand-specific RNA synthesis complexes. In some constructs, the partial reestablishment of PV1 amino acid sequences in this region was capable of rescuing RNA replication in vitro and in cell culture.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.