ArticleGenes & development2004
Conversion of myoblasts to physiologically active neuronal phenotype.
Article in Genes & development, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
32 citing papers in PubMed, 76 citations in OpenAlex.
- The Dynamic Roles of Repressor Element 1-Silencing Transcription Factor (REST): A Double-Edged Sword in Neural Health and Disease.Molecular neurobiology · 2025Review
- APOBEC2 safeguards skeletal muscle cell fate through binding chromatin and regulating transcription of non-muscle genes during myoblast differentiation.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- REST Is Not Resting: REST/NRSF in Health and Disease.Biomolecules · 2023Review
- REST/NRSF Silencing Modifies Neuronal Gene Expression in siRNA-Treated HeLa Cells: A Preliminary Exploration in the Search for Neuronal Biomarkers of Cervical Cancer.Medicina (Kaunas, Lithuania) · 2023Article
- Induction of an immortalized songbird cell line allows for gene characterization and knockout by CRISPR-Cas9.Scientific reports · 2022Article
- The SMYD1 and skNAC transcription factors contribute to neurodegenerative diseases.Brain, behavior, & immunity - health · 2020Article
- REST-DRD2 mechanism impacts glioblastoma stem cell-mediated tumorigenesis.Neuro-oncology · 2019Article
- miR-124-3p is a chronic regulator of gene expression after brain injury.Cellular and molecular life sciences : CMLS · 2018Article
- Polypyrimidine tract-binding protein blocks miRNA-124 biogenesis to enforce its neuronal-specific expression in the mouse.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- REST overexpression in mice causes deficits in spontaneous locomotion.Scientific reports · 2018Article
- Holding of bovine blastocysts at suprazero temperatures using small molecules.Scientific reports · 2017Article
- Induced dopaminergic neurons: A new promise for Parkinson's disease.Redox biology · 2017Article
- Brain REST/NRSF Is Not Only a Silent Repressor but Also an Active Protector.Molecular neurobiology · 2017Review
- Direct conversion of fibroblasts to neurons by reprogramming PTB-regulated microRNA circuits.Cell · 2013Article
- A case of cellular alchemy: lineage reprogramming and its potential in regenerative medicine.Journal of molecular cell biology · 2012Review
- Genome-wide recruitment to Polycomb-modified chromatin and activity regulation of the synovial sarcoma oncogene SYT-SSX2.BMC genomics · 2012Article
- Reprogramming of mesenchymal stem cells by the synovial sarcoma-associated oncogene SYT-SSX2.Oncogene · 2012Article
- Neuronal transcriptional repressor REST suppresses an Atoh7-independent program for initiating retinal ganglion cell development.Developmental biology · 2011Article
- Myoblast-derived neuronal cells form glutamatergic neurons in the mouse cerebellum.Stem cells (Dayton, Ohio) · 2010Article
- The transcriptional repressor REST/NRSF modulates hedgehog signaling.Developmental biology · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
Abstract
Repressor element 1 (RE1)-silencing transcription factor (REST)/neuron-restrictive silencer factor (NRSF) can repress several terminal neuronal differentiation genes by binding to a specific DNA sequence (RE1/neuron-restrictive silencer element [NRSE]) present in their regulatory regions. REST-VP16 binds to the same RE1/NRSE, but activates these REST/NRSF target genes. However, it is unclear whether REST-VP16 expression is sufficient to cause formation of functional neurons either from neural stem cells or from heterologous stem cells. Here we show that the expression of REST-VP16 in myoblasts grown under muscle differentiation conditions blocked entry into the muscle differentiation pathway, countered endogenous REST/NRSF-dependent repression, activated the REST/NRSF target genes, and, surprisingly, activated other neuronal differentiation genes and converted the myoblasts to a physiologically active neuronal phenotype. Furthermore, in vitro differentiated neurons produced by REST-VP16-expressing myoblasts, when injected into mouse brain, survived, incorporated into the normal brain, and did not form tumors. This is the first instance in which myoblasts were converted to a neuronal phenotype. Our results suggest that direct activation of REST/NRSF target genes with a single transgene, REST-VP16, is sufficient to activate other terminal neuronal differentiation genes and to override the muscle differentiation pathways, and they suggest that this approach provides an efficient way of triggering neuronal differentiation in myoblasts and possibly other stem cells.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.