ArticleOncogene2004
Mitochondrial ceramide increases in UV-irradiated HeLa cells and is mainly derived from hydrolysis of sphingomyelin.
Article in Oncogene, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
What it found
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Who cites it
32 citing papers in PubMed, 81 citations in OpenAlex.
- Lipidomic assessment of the impact of Nannochloropsis oceanica microalga lipid extract on human skin keratinocytes exposed to chronic UVB radiation.Scientific reports · 2023Article
- Anticancer Effects of New Ceramides Isolated from the Red Sea Red AlgaeMarine drugs · 2022Article
- Changes in Lipid Profile of Keratinocytes from Rat Skin Exposed to Chronic UVA or UVB Radiation and Topical Application of Cannabidiol.Antioxidants (Basel, Switzerland) · 2020Article
- Ceramide/Sphingomyelin Rheostat Regulated by Sphingomyelin Synthases and Chronic Diseases in Murine Models.Journal of lipid and atherosclerosis · 2020Review
- Actinoporins: From the Structure and Function to the Generation of Biotechnological and Therapeutic Tools.Biomolecules · 2020Review
- Article
- Aneuploid Cell Survival Relies upon Sphingolipid Homeostasis.Cancer research · 2017Article
- Ceramide-C16 Is a Versatile Modulator of Phosphatidylethanolamine Polymorphism.Biophysical journal · 2017Article
- Alkaline ceramidase 2 is a novel direct target of p53 and induces autophagy and apoptosis through ROS generation.Scientific reports · 2017Article
- Alkaline ceramidase 2 and its bioactive product sphingosine are novel regulators of the DNA damage response.Oncotarget · 2016Article
- Regulation of ceramide channel formation and disassembly: Insights on the initiation of apoptosis.Saudi journal of biological sciences · 2015Article
- Glycosphingolipids and cell death: one aim, many ways.Apoptosis : an international journal on programmed cell death · 2015Review
- Critical determinants of mitochondria-associated neutral sphingomyelinase (MA-nSMase) for mitochondrial localization.Biochimica et biophysica acta · 2015Article
- Evolving concepts in cancer therapy through targeting sphingolipid metabolism.Biochimica et biophysica acta · 2014Review
- PDMP induces rapid changes in vacuole morphology in Arabidopsis root cells.Journal of experimental botany · 2013Article
- Article
- Ablation of dihydroceramide desaturase confers resistance to etoposide-induced apoptosis in vitro.PloS one · 2012Article
- Novel pathway of ceramide production in mitochondria: thioesterase and neutral ceramidase produce ceramide from sphingosine and acyl-CoA.The Journal of biological chemistry · 2011Article
- Visualization of ceramide channels by transmission electron microscopy.Biochimica et biophysica acta · 2011Article
- Ceramide and mitochondria in ischemic brain injury.International journal of biochemistry and molecular biology · 2011Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Sphingolipids are important signaling molecules in many biologic processes, but little is known about their organelle-specific roles. Using HeLa cells, we investigated the effects of UV and etoposide-induced apoptosis on the contents of sphingomyelin (SM) and ceramide in subcellular compartments. UV irradiation of HeLa cells increased the levels of SM in all subcellular fractions, but the change was most dramatic in mitochondria. Using diacylglycerol kinase assays to quantify ceramide, we found that the levels of ceramide in mitochondria increased as early as 2 h after UV irradiation and remained elevated at 6 h. The increase in mitochondrial SM and ceramide was inhibited by D609, an inhibitor of sphingomyelinase and SM synthase. The inhibition of sphingolipid production correlated with protection of the mitochondrial transmembrane potential and prevention of cytochrome c release following UV irradiation. In contrast, myriocin, an inhibitor of the de novo ceramide synthesis pathway, only partially suppressed the production of ceramides in mitochondria and cannot suppress UV-induced apoptosis. Fumonicin B1, an inhibitor of ceramide synthase, can only prevent mitochondrial ceramide synthesis and UV-induced apoptosis in a small degree. These results indicate that mitochondrial ceramide production in UV-irradiated HeLa cells is not mediated by the de novo synthesis pathway, but mainly through SM hydrolysis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.