ArticlePlant physiology2004
Characterization of three functional high-affinity ammonium transporters in Lotus japonicus with differential transcriptional regulation and spatial expression.
Article in Plant physiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
29 citing papers in PubMed, 80 citations in OpenAlex.
- Nutrient-dependent regulation of symbiotic nitrogen fixation in legumes.Horticulture research · 2025Article
- Heat stress memory differentially regulates the expression of nitrogen transporter genes in the filamentous red alga 'Frontiers in plant science · 2024Article
- Identification and functional characterization of the sugarcane (Frontiers in plant science · 2023Article
- Sustaining nitrogen dynamics: A critical aspect for improving salt tolerance in plants.Frontiers in plant science · 2023Review
- Functional characterization of the sugarcane (Frontiers in plant science · 2022Article
- Nitrogen and Phosphorus Signaling and Transport During Legume-Rhizobium Symbiosis.Frontiers in plant science · 2021Review
- Combined Transcriptome and Proteome Analysis of Masson Pine (International journal of molecular sciences · 2020Article
- Function and Regulation of Ammonium Transporters in Plants.International journal of molecular sciences · 2020Review
- Cloning and Functional Determination of Ammonium Transporter PpeAMT3;4 in Peach.BioMed research international · 2020Article
- Fern genomes elucidate land plant evolution and cyanobacterial symbioses.Nature plants · 2018Article
- Identification and characterization of two ammonium transporter genes in flowering Chinese cabbage (Plant biotechnology (Tokyo, Japan) · 2018Article
- The AMT1 family genes from Malus robusta display differential transcription features and ammonium transport abilities.Molecular biology reports · 2017Article
- Wheat Ammonium Transporter (AMT) Gene Family: Diversity and Possible Role in Host-Pathogen Interaction with Stem Rust.Frontiers in plant science · 2017Article
- Gene characterization and transcription analysis of two new ammonium transporters in pear rootstock (Pyrus betulaefolia).Journal of plant research · 2016Article
- Influence of different host associations on glutamine synthetase activity and ammonium transporter inPhysiology and molecular biology of plants : an international journal of functional plant biology · 2016Article
- Article
- The rice OsAMT1;1 is a proton-independent feedback regulated ammonium transporter.Plant cell reports · 2015Article
- A nitrogen-dependent switch in the high affinity ammonium transport in Medicago truncatula.Plant molecular biology · 2014Article
- Soybean SAT1 (Symbiotic Ammonium Transporter 1) encodes a bHLH transcription factor involved in nodule growth and NH4+ transport.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Evolutionary classification of ammonium, nitrate, and peptide transporters in land plants.BMC evolutionary biology · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
12 authors at 4 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Ammonium is a primary source of nitrogen for plants. In legume plants ammonium can also be obtained by symbiotic nitrogen fixation, and NH(4)(+) is also a regulator of early and late symbiotic interaction steps. Ammonium transporters are likely to play important roles in the control of nodule formation as well as in nitrogen assimilation. Two new genes, LjAMT1;2 and LjAMT1;3, were cloned from Lotus japonicus. Both were able to complement the growth defect of a yeast (Saccharomyces cerevisiae) ammonium transport mutant. Measurement of [(14)C]methylammonium uptake rates and competition experiments revealed that each transporter had a high affinity for NH(4)(+). The K(i) for ammonium was 1.7, 3, and 15 microm for LjAMT1;1, 1;2, and 1;3, respectively. Real-time PCR revealed higher expression of LjAMT1;1, 1;2, and 1;3 genes in leaves than in roots and nodule, with expression levels decreasing in the order LjAMT1;1 > 1;2 > 1;3 except in flowers, in which LjAMT1;3 was expressed at higher level than in leaves, and LjAMT1;1 showed the lowest level of expression. Expression of LjAMT1;1 and 1;2 in roots was induced by nitrogen deprivation. Expression of LjAMT1;1 was repressed in leaves exposed to elevated CO(2) concentrations, which also suppress photorespiration. Tissue and cellular localization of LjAMT1 genes expression, using promoter-beta-glucuronidase and in situ RNA hybridization approaches, revealed distinct cellular spatial localization in different organs, including nodules, suggesting differential roles in the nitrogen metabolism of these organs.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.