ArticleMolecular biology of the cell2004
Role of the midbody matrix in cytokinesis: RNAi and genetic rescue analysis of the mammalian motor protein CHO1.
Article in Molecular biology of the cell, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
What it found
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Who cites it
31 citing papers in PubMed.
- The midbody and midbody remnant: from cellular debris to signaling organelle with diagnostic and therapeutic potential.Molecular biology of the cell · 2025Review
- The mammalian midbody and midbody remnant are assembly sites for RNA and localized translation.Developmental cell · 2023Article
- Article
- A Ruthenium(II) Polypyridyl Complex Disrupts Actin Cytoskeleton Assembly and Blocks Cytokinesis.Angewandte Chemie (Weinheim an der Bergstrasse, Germany) · 2022Article
- A Ruthenium(II) Polypyridyl Complex Disrupts Actin Cytoskeleton Assembly and Blocks Cytokinesis.Angewandte Chemie (International ed. in English) · 2022Article
- KIF5B modulates central spindle organization in late-stage cytokinesis in chondrocytes.Cell & bioscience · 2019Article
- Dynamic Phosphorylation of NudC by Aurora B in Cytokinesis.PloS one · 2016Article
- Phosphorylation of CHO1 by Lats1/2 regulates the centrosomal activation of LIMK1 during cytokinesis.Cell cycle (Georgetown, Tex.) · 2015Article
- Binding of Kif23-iso1/CHO1 to 14-3-3 is regulated by sequential phosphorylations at two LATS kinase consensus sites.PloS one · 2015Article
- SHCBP1 is required for midbody organization and cytokinesis completion.Cell cycle (Georgetown, Tex.) · 2014Article
- Rho GTPases as regulators of mitosis and cytokinesis in mammalian cells.Small GTPases · 2014Review
- The nuclear F-actin interactome of Xenopus oocytes reveals an actin-bundling kinesin that is essential for meiotic cytokinesis.The EMBO journal · 2013Article
- ARF6 GTPase protects the post-mitotic midbody from 14-3-3-mediated disintegration.The EMBO journal · 2012Article
- Midbody assembly and its regulation during cytokinesis.Molecular biology of the cell · 2012Article
- Germ cell intercellular bridges.Cold Spring Harbor perspectives in biology · 2011Review
- Syndecan-4 promotes cytokinesis in a phosphorylation-dependent manner.Cellular and molecular life sciences : CMLS · 2010Article
- Kinesins to the core: The role of microtubule-based motor proteins in building the mitotic spindle midzone.Seminars in cell & developmental biology · 2010Review
- Mutations in a gene encoding a midbody protein in binucleated Reed-Sternberg cells of Hodgkin lymphoma.Cell cycle (Georgetown, Tex.) · 2010Article
- Understanding cytokinesis failure.Advances in experimental medicine and biology · 2010Review
- Mouse TEX14 is required for embryonic germ cell intercellular bridges but not female fertility.Biology of reproduction · 2009Article
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
CHO1 is a kinesin-like motor protein essential for cytokinesis in mammalian cells. To analyze how CHO1 functions, we established RNAi and genetic rescue assays. CHO1-depleted cells reached a late stage of cytokinesis but fused back to form binucleate cells because of the absence of the midbody matrix in the middle of the intercellular bridge. Expression of exogenous CHO1 restored the formation of the midbody matrix and rescued cytokinesis in siRNA-treated cells. By analyzing phenotypes rescued with different constructs, it was shown that both motor and stalk domains function in midbody formation, whereas the tail is essential for completion of cytokinesis after the midbody matrix has formed. During the terminal stage of cytokinesis, different subregions of the tail play distinctive roles in stabilizing the midbody matrix and maintaining an association between the midbody and cell cortex. These results demonstrate that CHO1 consists of functionally differentiated subregions that act in concert to ensure complete cell separation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.