ArticleEukaryotic cell2004
Cryptosporidium parvum mitochondrial-type HSP70 targets homologous and heterologous mitochondria.
Article in Eukaryotic cell, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
13 citing papers in PubMed, 61 citations in OpenAlex.
- The history of cryptosporidiosis.Parasites & vectors · 2026Review
- Implication of Potential Differential Roles of the Two Phosphoglucomutase Isoforms in the Protozoan ParasitePathogens (Basel, Switzerland) · 2021Article
- The Elusive Mitochondrial Genomes of Apicomplexa: Where Are We Now?Frontiers in microbiology · 2021Review
- The Role of Hsp70s in the Development and Pathogenicity of Plasmodium falciparum.Advances in experimental medicine and biology · 2021Article
- Localization of Fe-S Biosynthesis Machinery in Cryptosporidium parvum Mitosome.The Journal of eukaryotic microbiology · 2018Article
- Diversity and reductive evolution of mitochondria among microbial eukaryotes.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2010Review
- Mitochondrion-related organelles in eukaryotic protists.Annual review of microbiology · 2010Review
- Mitochondria in malaria and related parasites: ancient, diverse and streamlined.Journal of bioenergetics and biomembranes · 2008Review
- Identification and characterization of a mitochondrial iron-superoxide dismutase of Cryptosporidium parvum.Parasitology research · 2008Article
- The structural and functional diversity of Hsp70 proteins from Plasmodium falciparum.Protein science : a publication of the Protein Society · 2007Review
- Frataxin, a conserved mitochondrial protein, in the hydrogenosome of Trichomonas vaginalis.Eukaryotic cell · 2007Article
- Stable expression of Cryptosporidium parvum glycoprotein gp40/15 in Toxoplasma gondii.Molecular and biochemical parasitology · 2007Article
- Multiple secondary origins of the anaerobic lifestyle in eukaryotes.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2006Review
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
Abstract
A mitochondrial HSP70 gene (Cp-mtHSP70) is described for the apicomplexan Cryptosporidium parvum, an agent of diarrhea in humans and animals. Mitochondrial HSP70 is known to have been acquired from the proto-mitochondrial endosymbiont. The amino acid sequence of Cp-mtHSP70 shares common domains with mitochondrial and proteobacterial homologues, including 34 amino acids of an NH2-terminal mitochondrion-like targeting presequence. Phylogenetic reconstruction places Cp-mtHSP70 within the mitochondrial clade of HSP70 homologues. Using reverse transcription-PCR, Cp-mtHSP70 mRNA was observed in C. parvum intracellular stages cultured in HCT-8 cells. Polyclonal antibodies to Cp-mtHSP70 recognize a approximately 70-kDa protein in Western blot analysis of sporozoite extracts. Both fluorescein- and immunogold-labeled anti-Cp-mtHSP70 localize to a single mitochondrial compartment in close apposition to the nucleus. Furthermore, the NH2-terminal presequence of Cp-mtHSP70 can correctly target green fluorescent protein to the single mitochondrion of the apicomplexan Toxoplasma gondii and the mitochondrial network of the yeast Saccharomyces cerevisiae. When this presequence was truncated, the predicted amphiphilic alpha-helix was shown to be essential for import into the yeast mitochondrion. These data further support the presence of a secondarily reduced relict mitochondrion in C. parvum.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.