ArticleEukaryotic cell2004
Polarized hyphal growth in Candida albicans requires the Wiskott-Aldrich Syndrome protein homolog Wal1p.
Article in Eukaryotic cell, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 55 citations in OpenAlex.
- Exploring roles for essential proteins in yeast filamentous growth identifies the WASP homolog Las17 as a regulator of the Cdc42-dependent fMAPK pathway.bioRxiv : the preprint server for biology · 2026Article
- Endocytosis Mediated byMicroorganisms · 2026Article
- The Early Endocytosis GeneJournal of fungi (Basel, Switzerland) · 2023Article
- Surface Architecture Influences the Rigidity ofNanomaterials (Basel, Switzerland) · 2022Article
- Effects of Hst3p inhibition inFrontiers in cellular and infection microbiology · 2022Article
- From Jekyll to Hyde: The Yeast-Hyphal Transition ofPathogens (Basel, Switzerland) · 2021Review
- The regulation of hyphae growth inVirulence · 2020Review
- The Role of Secretory Pathways inJournal of fungi (Basel, Switzerland) · 2020Review
- Article
- Role of clathrin-mediated endocytosis in the use of heme and hemoglobin by the fungal pathogen Cryptococcus neoformans.Cellular microbiology · 2019Article
- 14-Helical β-Peptides Elicit Toxicity against C. albicans by Forming Pores in the Cell Membrane and Subsequently Disrupting Intracellular Organelles.Cell chemical biology · 2019Article
- A Global Analysis of Kinase Function inFrontiers in cellular and infection microbiology · 2018Article
- Chemical inhibitors of Candida albicans hyphal morphogenesis target endocytosis.Scientific reports · 2017Article
- Mapping the Hsp90 Genetic Network Reveals Ergosterol Biosynthesis and Phosphatidylinositol-4-Kinase Signaling as Core Circuitry Governing Cellular Stress.PLoS genetics · 2016Article
- Plasma membrane organization promotes virulence of the human fungal pathogen Candida albicans.Journal of microbiology (Seoul, Korea) · 2016Review
- Endocytosis and early endosome motility in filamentous fungi.Current opinion in microbiology · 2014Review
- The spatial distribution of the exocyst and actin cortical patches is sufficient to organize hyphal tip growth.Eukaryotic cell · 2013Article
- Wsp1 is downstream of Cin1 and regulates vesicle transport and actin cytoskeleton as an effector of Cdc42 and Rac1 in Cryptococcus neoformans.Eukaryotic cell · 2012Article
- Actin organization and dynamics in filamentous fungi.Nature reviews. Microbiology · 2011Review
- Growth of Candida albicans hyphae.Nature reviews. Microbiology · 2011Review
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The yeast-to-hypha transition is a key feature in the cell biology of the dimorphic human fungal pathogen Candida albicans. Reorganization of the actin cytoskeleton is required for this dimorphic switch in Candida. We show that C. albicans WAL1 mutants with both copies of the Wiskott-Aldrich syndrome protein (WASP) homolog deleted do not form hyphae under all inducing conditions tested. Growth of the wild-type and wal1 mutant strains was monitored by in vivo time-lapse microscopy both during yeast-like growth and under hypha-inducing conditions. Isotropic bud growth produced round wal1 cells and unusual mother cell growth. Defects in the organization of the actin cytoskeleton resulted in the random localization of actin patches. Furthermore, wal1 cells exhibited defects in the endocytosis of the lipophilic dye FM4-64, contained increased numbers of vacuoles compared to the wild type, and showed defects in bud site selection. Under hypha-inducing conditions wal1 cells were able to initiate polarized morphogenesis, which, however, resulted in the formation of pseudohyphal cells. Green fluorescent protein (GFP)-tagged Wal1p showed patch-like localization in emerging daughter cells during the yeast growth phase and at the hyphal tips under hypha-inducing conditions. Wal1p-GFP localization largely overlapped with that of actin. Our results demonstrate that Wal1p is required for the organization of the actin cytoskeleton and hyphal morphogenesis in C. albicans as well as for endocytosis and vacuole morphology.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.