ArticleEuropean biophysics journal : EBJ2004
Fast vesicle transport in PC12 neurites: velocities and forces.
Article in European biophysics journal : EBJ, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 55 papers.
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Who cites it
55 citing papers in PubMed, 142 citations in OpenAlex.
- Impact of physiological ionic strength and crowding on kinesin-1 motility.Cell structure and function · 2025Article
- Review
- A kinesin-1 adaptor complex controls bimodal slow axonal transport of spectrin in Caenorhabditis elegans.Developmental cell · 2023Article
- Kinesin-1 transports morphologically distinct intracellular virions during vaccinia infection.Journal of cell science · 2023Article
- Effects of dynein inhibitor on the number of motor proteins transporting synaptic cargos.Biophysical journal · 2021Article
- Chemogenetic Manipulation of Dopamine Neurons Dictates Cocaine Potency at Distal Dopamine Transporters.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2020Article
- The Impact of Rate Formulations on Stochastic Molecular Motor Dynamics.Scientific reports · 2019Article
- Geometry of antiparallel microtubule bundles regulates relative sliding and stalling by PRC1 and Kif4A.eLife · 2018Article
- Non-invasive Neurite Mechanics in Differentiated PC12 Cells.Frontiers in cellular neuroscience · 2018Article
- Small teams of myosin Vc motors coordinate their stepping for efficient cargo transport on actin bundles.The Journal of biological chemistry · 2017Article
- Transport efficiency of membrane-anchored kinesin-1 motors depends on motor density and diffusivity.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Interrogating Emergent Transport Properties for Molecular Motor Ensembles: A Semi-analytical Approach.PLoS computational biology · 2016Article
- The Dark Matter of Biology.Biophysical journal · 2016Review
- Dividing organelle tracks into Brownian and motor-driven intervals by variational maximization of the Bayesian evidence.European biophysics journal : EBJ · 2016Article
- Automated Multi-Peak Tracking Kymography (AMTraK): A Tool to Quantify Sub-Cellular Dynamics with Sub-Pixel Accuracy.PloS one · 2016Article
- Nanoparticle-assisted optical tethering of endosomes reveals the cooperative function of dyneins in retrograde axonal transport.Scientific reports · 2015Article
- Navigating the plant cell: intracellular transport logistics in the green kingdom.Molecular biology of the cell · 2015Article
- Highly loaded behavior of kinesins increases the robustness of transport under high resisting loads.PLoS computational biology · 2015Article
- Motor coupling through lipid membranes enhances transport velocities for ensembles of myosin Va.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Drag of the cytosol as a transport mechanism in neurons.Biophysical journal · 2014Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Although the mechanical behavior of single-motor protein molecules such as kinesin has been carefully studied in buffer, the mechanical behavior of motor-driven vesicles in cells is much less understood. We have tracked single vesicles in neurites of PC12 cells with a spatial precision of +/-30 nm and a time resolution of 120 ms. Because the neurites are thin, long, straight, and attached to the surface of planar cover glasses, the velocity of individual vesicles could be measured for times as long as 15 s and distances as long as 15 mum. The velocity of anterograde vesicles was in most cases constant for periods of 1-2 s, then changed in a step-like fashion to a new constant velocity. The viscoelastic modulus felt by the vesicles within live PC12 cells was determined from the Brownian motion, using Mason's generalization of the Stokes-Einstein equation. From Stokes' law, the drag force at the smallest sustained velocity was 4.2+/-0.6 pN for vesicles of radius 0.30-0.40 mum, about half the maximum force which conventional kinesin can develop during bead assays in buffer. We interpret the observed velocity steps as changes of +/-1 or occasionally +/-2 in the number of active motor proteins dragging that vesicle along a microtubule. Assuming that the motor is conventional kinesin, which hydrolyzes one ATP per 8 nm step along the microtubule, the motor protein efficiency in PC12 neurites is approximately 35%.
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