ArticleCurrent genetics2004
Establishment of mRFP1 as a fluorescent marker in Aspergillus nidulans and construction of expression vectors for high-throughput protein tagging using recombination in vitro (GATEWAY).
Article in Current genetics, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
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Who cites it
47 citing papers in PubMed, 110 citations in OpenAlex.
- The photolyase/cryptochrome of Aspergillus nidulans senses oxidative stress and shuttles from nuclei to mitochondria.Nature communications · 2026Article
- Distinct roles of phytochromes A and B inmBio · 2025Article
- An ascomycete H4 variant with an unknown function.Royal Society open science · 2024Article
- Article
- Golden Gate vectors for efficient gene fusion and gene deletion in diverse filamentous fungi.Current genetics · 2021Article
- Establishment of the monomeric yellow-green fluorescent protein mNeonGreen for life cell imaging in mycelial fungi.AMB Express · 2020Article
- Fluorescent protein expression in the ectomycorrhizal fungus Laccaria bicolor: a plasmid toolkit for easy use of fluorescent markers in basidiomycetes.Current genetics · 2020Article
- Invasive growth ofFungal biology and biotechnology · 2020Article
- Nup2 performs diverse interphase functions in Aspergillus nidulans.Molecular biology of the cell · 2018Article
- Cyclins in aspergilli: Phylogenetic and functional analyses of group I cyclins.Studies in mycology · 2018Article
- Mitotic nuclear pore complex segregation involves Nup2 inThe Journal of cell biology · 2017Article
- Tools for retargeting proteins within Aspergillus nidulans.PloS one · 2017Article
- Fungi use the SakA (HogA) pathway for phytochrome-dependent light signalling.Nature microbiology · 2016Article
- Effect of secretory pathway gene overexpression on secretion of a fluorescent reporter protein inFungal biology and biotechnology · 2016Article
- The Adenylate-Forming Enzymes AfeA and TmpB Are Involved in Aspergillus nidulans Self-Communication during Asexual Development.Frontiers in microbiology · 2016Article
- Overexpression of the tomato pollen receptor kinase LePRK1 rewires pollen tube growth to a blebbing mode.The Plant cell · 2014Article
- Modelling and mutational analysis of Aspergillus nidulans UreA, a member of the subfamily of urea/H⁺ transporters in fungi and plants.Open biology · 2014Article
- The NIMA kinase is required to execute stage-specific mitotic functions after initiation of mitosis.Eukaryotic cell · 2014Article
- Aspergillus nidulans Pmts form heterodimers in all pairwise combinations.FEBS open bio · 2014Article
- The cell-end marker TeaA and the microtubule polymerase AlpA contribute to microtubule guidance at the hyphal tip cortex of Aspergillus nidulans to provide polarity maintenance.Journal of cell science · 2013Article
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The advent of fluorescent proteins as vital dyes had a major impact in many research fields. Different green fluorescent protein (GFP) variants were established in prokaryotic and eukaryotic organisms within the past 10 years, and other fluorescent proteins were discovered and applied. We expressed the Discosoma red fluorescent protein, DsRed (T4), the improved monomeric red fluorescent protein (mRFP1) and the blue fluorescent protein (BFP) in the filamentous fungus Aspergillus nidulans. Whereas DsRed requires tetramer formation for fluorescence, mRFP1 functions as monomer. We used sGFP, DsRed (T4), mRFP1 and BFP for nuclear and/or mitochondrial labelling. To facilitate gene tagging, we established a number of cloning vectors for the efficient, simultaneous fusion of any protein with mRFP1, BFP and sGFP or the haemagglutinin epitope, 3xHA. A PCR-amplified gene of interest can be inserted into the expression vectors without cloning but using homologous recombination in vitro (GATEWAY). The vectors contain the argB gene as a selection marker for A. nidulans and the inducible alcA promoter for control of expression. The system allows labelling of a protein with several tags in one recombination reaction. Both the nutritional marker gene and the promoter are frequently used in other fungi, suggesting that this set of expression vectors will be very useful tools for gene analysis on a genome-wide scale.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.