ArticleEMBO reports2004
The absence of the yeast chromatin assembly factor Asf1 increases genomic instability and sister chromatid exchange.
Article in EMBO reports, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 58 papers.
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Who cites it
58 citing papers in PubMed, 110 citations in OpenAlex.
- A Rfa1-MN-based system reveals new factors involved in the rescue of broken replication forks.PLoS genetics · 2025Article
- Multifaceted roles of H2B mono-ubiquitylation in D-loop metabolism during homologous recombination repair.Nucleic acids research · 2025Article
- Unraveling the Role of JMJD1B in Genome Stability and the Malignancy of Melanomas.International journal of molecular sciences · 2024Article
- Defective transfer of parental histone decreases frequency of homologous recombination by increasing free histone pools in budding yeast.Nucleic acids research · 2024Article
- Article
- PIF7-mediated epigenetic reprogramming promotes the transcriptional response to shade in Arabidopsis.The EMBO journal · 2023Article
- Histone acetylation dynamics in repair of DNA double-strand breaks.Frontiers in genetics · 2022Review
- Parental nucleosome segregation and the inheritance of cellular identity.Nature reviews. Genetics · 2021Review
- JMJD1B, a novel player in histone H3 and H4 processing to ensure genome stability.Epigenetics & chromatin · 2020Article
- Article
- Molecular basis of Tousled-Like Kinase 2 activation.Nature communications · 2018Article
- Review
- Budding Yeast Rif1 Controls Genome Integrity by Inhibiting rDNA Replication.PLoS genetics · 2016Article
- Article
- Influence of Human p53 on Plant Development.PloS one · 2016Article
- Functions of Ubiquitin and SUMO in DNA Replication and Replication Stress.Frontiers in genetics · 2016Review
- The chromatin assembly factor 1 promotes Rad51-dependent template switches at replication forks by counteracting D-loop disassembly by the RecQ-type helicase Rqh1.PLoS biology · 2014Article
- Genome-wide mapping of yeast histone chaperone anti-silencing function 1 reveals its role in condensin binding with chromatin.PloS one · 2014Article
- ANTI-SILENCING FUNCTION1 proteins are involved in ultraviolet-induced DNA damage repair and are cell cycle regulated by E2F transcription factors in Arabidopsis.Plant physiology · 2013Article
- Rtt109 prevents hyper-amplification of ribosomal RNA genes through histone modification in budding yeast.PLoS genetics · 2013Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Histone chaperone Asf1 participates in heterochromatin silencing, DNA repair and regulation of gene expression, and promotes the assembly of DNA into chromatin in vitro. To determine the influence of Asf1 on genetic stability, we have analysed the effect of asf1Delta on homologous recombination. In accordance with a defect in nucleosome assembly, asf1Delta leads to a loss of negative supercoiling in plasmids. Importantly, asf1Delta increases spontaneous recombination between inverted DNA sequences. This increase correlates with an accumulation of double-strand breaks (DSBs) as determined by immunodetection of phosphorylated histone H2A and fluorescent detection of Rad52-YFP foci during S and G2/M phases. In addition, asf1Delta shows high levels of sister chromatid exchange (SCE) and is proficient in DSB-induced SCE as determined by physical analysis. Our results suggest that defective chromatin assembly caused by asf1Delta leads to DSBs that can be repaired by SCE, affecting genetic stability.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.