ArticleJournal of clinical microbiology2004
Development of a multiplex PCR technique for detection and epidemiological typing of salmonella in human clinical samples.
Article in Journal of clinical microbiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 71 papers.
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Who cites it
71 citing papers in PubMed.
- ESBL- and pAmpC-ProducingAntibiotics (Basel, Switzerland) · 2026Article
- Activity of Lysozyme Against Multidrug-ResistantAnimals : an open access journal from MDPI · 2025Article
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- Multi-locus sequence typing of Salmonella enterica isolates from dog treats and raw meat-based dog food in Japan.BMC research notes · 2024Article
- Prevalence, Molecular Detection, and Antimicrobial Resistance ofMicroorganisms · 2024Article
- Assessment of multidrug-resistant profile, multi-locus sequence typing and efflux pump activity in Salmonella Typhimurium isolated from hospital sewage.World journal of microbiology & biotechnology · 2023Article
- Antimicrobial Susceptibility Profile of Pathogenic and Commensal Bacteria Recovered from Cattle and Goat Farms.Antibiotics (Basel, Switzerland) · 2023Article
- Anti-microbial resistance of Salmonella isolates from raw meat-based dog food in Japan.Veterinary medicine and science · 2022Article
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- Molecular detection ofIranian journal of veterinary research · 2022Article
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- Prevalence, typing and antimicrobial resistance of Salmonella isolates from commercial shellfish in the North coast of Morocco.World journal of microbiology & biotechnology · 2021Article
- Virulence and Antimicrobial Resistance Profiles ofMicroorganisms · 2021Article
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- Development of a Genoserotyping Method forMicroorganisms · 2020Article
- Prevalence of Multidrug-Resistant Foodborne Pathogens and Indicator Bacteria from Edible Offal and Muscle Meats in Nashville, Tennessee.Foods (Basel, Switzerland) · 2020Article
- Molecular detection of virulence genes in Salmonella spp. isolated from chicken faeces in Mafikeng, South Africa.Journal of the South African Veterinary Association · 2020Article
- Enhanced Antibacterial Activity of Silver Nanoparticles Combined with Hydrogen Peroxide Against Multidrug-Resistant Pathogens Isolated from Dairy Farms and Beef Slaughterhouses in Egypt.Infection and drug resistance · 2020Article
- Detection ofFoods (Basel, Switzerland) · 2019Review
11 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have developed a multiplex PCR assay for Salmonella detection and epidemiological typing. Six sets of primers were designed to detect the major Salmonella serotypes and phage types in Spain. An internal amplification control was designed in order to detect PCR inhibition. The different amplification profiles obtained allowed us to detect Salmonella bacteria and to distinguish the clinically prevalent Salmonella enterica serotypes Enteritidis, Typhimurium and subspecies I serotype 4,5,12:i:-. Using this method, we could detect a specific band for DT104 and U302 phage types in Salmonella serotype Typhimurium. Salmonella enterica serotype Hadar and other C2 serogroup strains showed two specific band profiles. In the validation stage, the assay was reproducible for all serotypes studied, apart from some C2 serogroup strains. When the technique was applied to clinical stool specimens, the prevalent serotypes Enteritidis and Typhimurium were detected with a sensitivity of 93%, specificity of 100%, and efficiency of 98%. Also, a low PCR inhibition rate (8%) was obtained. The overall agreement of the multiplex PCR with conventional culture-based techniques was 95% for Salmonella typing using Cohen's kappa index.
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