ArticleJournal of clinical microbiology2004
Comparison of two widely used PCR primer systems for detection of toxoplasma in amniotic fluid, blood, and tissues.
Article in Journal of clinical microbiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers, 1 of them a synthesis that pooled it.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
28 citing papers in PubMed, 1 synthesis or guideline pooled it, 84 citations in OpenAlex.
- Prenatal education for congenital toxoplasmosis.The Cochrane database of systematic reviews · 2015Pooled it
- Systematic Review and Meta-Analysis of Congenital Toxoplasmosis Diagnosis: Advances and Challenges.Journal of tropical medicine · 2024Review
- Comparison of real-time PCR and nested PCR for toxoplasmosis diagnosis in toxoplasmic retinochoroiditis patients.BMC infectious diseases · 2021Article
- Enhancing the detection ofBiotechnology reports (Amsterdam, Netherlands) · 2019Article
- Inhibition of polymerase chain reaction: Pathogen-specific controls are better than human gene amplification.PloS one · 2019Article
- Seromolecular assess ofEXCLI journal · 2019Article
- Performance of 2 Polymerization Protocols Targeting Cloned Toxoplasma Parasites.Open access Macedonian journal of medical sciences · 2018Article
- Pathology, clinical signs, and tissue distribution ofInternational journal for parasitology. Parasites and wildlife · 2017Article
- Toxoplasma gondii Infection and Chronic Liver Diseases: Evidence of an Association.Tropical medicine and infectious disease · 2016Article
- A 10-year retrospective comparison of two target sequences, REP-529 and B1, for Toxoplasma gondii detection by quantitative PCR.Journal of clinical microbiology · 2015Article
- Multicentric comparative assessment of the bio-evolution Toxoplasma gondii detection kit with eight laboratory-developed PCR assays for molecular diagnosis of congenital toxoplasmosis.Journal of clinical microbiology · 2015Article
- Characterization and multicentric validation of a common standard for Toxoplasma gondii detection using nucleic acid amplification assays.Journal of clinical microbiology · 2014Article
- Article
- Comparative assessment of a commercial kit and two laboratory-developed PCR assays for molecular diagnosis of congenital toxoplasmosis.Journal of clinical microbiology · 2012Article
- Novel interpretation of molecular diagnosis of congenital toxoplasmosis according to gestational age at the time of maternal infection.Journal of clinical microbiology · 2012Article
- Real-time RT-PCR on SAG1 and BAG1 gene expression during stage conversion in immunosuppressed mice infected with Toxoplasma gondii Tehran strain.The Korean journal of parasitology · 2012Article
- Multicentric comparative analytical performance study for molecular detection of low amounts of Toxoplasma gondii from simulated specimens.Journal of clinical microbiology · 2010Article
- Serological prevalence of Toxoplasma gondii antibodies in pregnant women from Southern Brazil.Parasitology research · 2010Article
- Multicenter comparative evaluation of five commercial methods for toxoplasma DNA extraction from amniotic fluid.Journal of clinical microbiology · 2009Article
- Molecular diagnosis of Toxoplasma gondii infection in cerebrospinal fluid from AIDS patients.Cerebrospinal fluid research · 2009Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The PCR diagnosis of toxoplasmosis suffers from lack of standardization. Interlaboratory comparative studies of PCR methods have been performed, but intralaboratory comparisons are scarce. Here, we optimized and compared the technical performances of two PCR primer systems widely used for Toxoplasma detection. The differences between the two methods were visible only at low parasite concentrations (< or = 1 Toxoplasma genome per reaction tube). Nevertheless, when clinical samples were tested, both methods significantly differed in their technical sensitivities and specificities. Only one method appeared adequate for samples containing blood or tissue.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.