ArticleJournal of clinical microbiology2004
Simultaneous detection of marine fish pathogens by using multiplex PCR and a DNA microarray.
Article in Journal of clinical microbiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 132 citations in OpenAlex.
- Development of a Multiplex PCR Assay for Simultaneous Identification of Six Commercially Important Bivalves.Foods (Basel, Switzerland) · 2025Article
- Fluorescence-based spectrometric and imaging methods and machine learning analyses for microbiota analysis.Mikrochimica acta · 2025Review
- Occurrence ofVeterinary world · 2020Article
- Simultaneous Identification of Ten Bacterial Pathogens Using the Multiplex Ligation Reaction Based on the Probe Melting Curve Analysis.Scientific reports · 2017Article
- Monitoring and managing microbes in aquaculture - Towards a sustainable industry.Microbial biotechnology · 2016Review
- Severe Wound Infection with Photobacterium damselae ssp. damselae and Vibrio harveyi, following a Laceration Injury in Marine Environment: A Case Report and Review of the Literature.Case reports in medicine · 2013Article
- Simultaneous detection of multiple fish pathogens using a naked-eye readable DNA microarray.Sensors (Basel, Switzerland) · 2012Article
- A New Generation Microarray for the Simultaneous Detection and Identification of Yersinia pestis and Bacillus anthracis in Food.Journal of pathogens · 2012Article
- Detection of representative enteropathogenic bacteria, Vibrio spp., pathogenic Escherichia coli, Salmonella spp., Shigella spp., and Yersinia enterocolitica, using a virulence factor gene-based oligonucleotide microarray.Journal of microbiology (Seoul, Korea) · 2010Article
- Detection of Enterobacter sakazakii and other pathogens associated with infant formula powder by use of a DNA microarray.Journal of clinical microbiology · 2009Article
- Robust detection and identification of multiple oomycetes and fungi in environmental samples by using a novel cleavable padlock probe-based ligation detection assay.Applied and environmental microbiology · 2009Article
- Microarray-based detection of viruses causing vesicular or vesicular-like lesions in livestock animals.Veterinary microbiology · 2009Article
- Nanoparticle diffraction gratings for DNA detection on photopatterned glass substrates.Biointerphases · 2008Article
- Food microbial pathogen detection and analysis using DNA microarray technologies.Foodborne pathogens and disease · 2008Article
- In situ-synthesized virulence and marker gene biochip for detection of bacterial pathogens in water.Applied and environmental microbiology · 2008Article
- Locked nucleic acid pentamers as universal PCR primers for genomic DNA amplification.PloS one · 2008Article
- Validation of mixed-genome microarrays as a method for genetic discrimination.Applied and environmental microbiology · 2007Article
- Suspension array analysis of 16S rRNA from Fe- and SO(4)2- reducing bacteria in uranium-contaminated sediments undergoing bioremediation.Applied and environmental microbiology · 2006Article
- Creating advanced multifunctional biosensors with surface enzymatic transformations.Langmuir : the ACS journal of surfaces and colloids · 2006Article
- A multiplex polymerase chain reaction microarray assay to detect bioterror pathogens in blood.The Journal of molecular diagnostics : JMD · 2005Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We coupled multiplex PCR and a DNA microarray to construct an assay suitable for the simultaneous detection of five important marine fish pathogens (Vibrio vulnificus, Listonella anguillarum, Photobacterium damselae subsp. damselae, Aeromonas salmonicida subsp. salmonicida, and Vibrio parahaemolyticus). The array was composed of nine short oligonucleotide probes (25-mer) complementary to seven chromosomal loci (cyt, rpoN, gyrB, toxR, ureC, dly, and vapA) and two plasmid-borne loci (fatA and A.sal). Nine primer sets were designed to amplify short fragments of these loci (100 to 177 bp) in a multiplex PCR. PCR products were subsequently labeled by nick translation and hybridized to the microarray. All strains of the five target species (n = 1 to 21) hybridized to at least one species-specific probe. Assay sensitivities ranged from 100% for seven probes to 83 and 67% for the two remaining probes. Multiplex PCR did not produce any nonspecific amplification products when tested against 23 related species of bacteria (n = 40 strains; 100% specificity). Using purified genomic DNA, we were able to detect PCR products with < 20 fg of genomic DNA per reaction (equivalent to four or five cells), and the array was at least fourfold more sensitive than agarose gel electrophoresis for detecting PCR products. In addition, our method allowed the tentative identification of virulent strains of L. anguillarum serotype O1 based on the presence of the fatA gene (67% sensitivity and 100% specificity). This assay is a sensitive and specific tool for the simultaneous detection of multiple pathogenic bacteria that cause disease in fish and humans.
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