ArticleJournal of clinical microbiology2004
Detection and genotyping of varicella-zoster virus by TaqMan allelic discrimination real-time PCR.
Article in Journal of clinical microbiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
24 citing papers in PubMed, 1 synthesis or guideline pooled it, 64 citations in OpenAlex.
- DNMT1, DNMT3A and DNMT3B Polymorphisms Associated With Gastric Cancer Risk: A Systematic Review and Meta-analysis.EBioMedicine · 2016Pooled it
- Prospective study of live attenuated vaccines for patients receiving immunosuppressive agents.PloS one · 2020Trial
- Article
- Sequence analysis of isolated strains of herpes zoster virus among patients with shingles.Iranian journal of microbiology · 2024Article
- Vaccine Strain and Wild-Type Clades of Varicella-Zoster Virus in Central Nervous System and Non-CNS Disease, New York State, 2004-2019.Journal of clinical microbiology · 2022Article
- Live attenuated vaccines under immunosuppressive agents or biological agents: survey and clinical data from Japan.European journal of pediatrics · 2021Article
- Real-time Detection and Monitoring of Loop Mediated Amplification (LAMP) Reaction Using Self-quenching and De-quenching Fluorogenic Probes.Scientific reports · 2018Article
- Arm Paralysis After Routine Childhood Vaccinations: Application of Advanced Molecular Methods to the Causality Assessment of an Adverse Event After Immunization.Journal of the Pediatric Infectious Diseases Society · 2017Article
- Microbiology laboratory and the management of mother-child varicella-zoster virus infection.World journal of virology · 2016Review
- Association between rs9930506 polymorphism of the fat mass & obesity-associated (FTO) gene & onset of obesity in Polish adults.The Indian journal of medical research · 2016Article
- Enzymatic Digestion Pattern of Varicella Zoster Virus ORF38 and ORF54 in Chickenpox Patients Using RFLP Technique.Iranian journal of pathology · 2016Article
- Two Anaplasma phagocytophilum strains in Ixodes scapularis ticks, Canada.Emerging infectious diseases · 2014Article
- Influence of survivin (BIRC5) and caspase-9 (CASP9) functional polymorphisms in renal cell carcinoma development: a study in a southern European population.Molecular biology reports · 2013Article
- Impact of varicella vaccine on varicella-zoster virus dynamics.Clinical microbiology reviews · 2010Review
- A real-time PCR assay to identify and discriminate among wild-type and vaccine strains of varicella-zoster virus and herpes simplex virus in clinical specimens, and comparison with the clinical diagnoses.Journal of medical virology · 2009Article
- Discriminating between varicella-zoster virus vaccine and wild-type strains by loop-mediated isothermal amplification.Journal of clinical microbiology · 2008Article
- Identification of five major and two minor genotypes of varicella-zoster virus strains: a practical two-amplicon approach used to genotype clinical isolates in Australia and New Zealand.Journal of virology · 2007Article
- Multiplex assay based on single-nucleotide polymorphisms for rapid identification of Brucella isolates at the species level.Applied and environmental microbiology · 2007Article
- Genotypic analysis of varicella-zoster virus and its seroprevalence in Finland.Clinical and vaccine immunology : CVI · 2007Article
- Toward universal varicella-zoster virus (VZV) genotyping: diversity of VZV strains from France and Spain.Journal of clinical microbiology · 2007Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A proportion of individuals vaccinated with live attenuated Oka varicella-zoster virus (VZV) vaccine subsequently develop attenuated chicken pox and/or herpes zoster. To determine whether postvaccination varicella infections are caused by vaccine or wild-type virus, a simple method for distinguishing the vaccine strain from wild-type virus is required. We have developed a TaqMan real-time PCR assay to detect and differentiate wild-type virus from Oka vaccine strains of VZV. The assay utilized two fluorogenic, minor groove binding probes targeted to a single nucleotide polymorphism in open reading frame 62 that distinguishes the Oka vaccine from wild-type strains. VZV DNA could be genotyped and quantified within minutes of thermocycling completion due to real-time monitoring of PCR product formation and allelic discrimination analysis. The allelic discrimination assay was performed in parallel with two standard PCR-restriction fragment length polymorphism (RFLP) methods on 136 clinical and laboratory VZV strains from Canada, Australia, and Japan. The TaqMan assay exhibited a genotyping accuracy of 100% and, when compared to both PCR-RFLP methods, was 100 times more sensitive. In addition, the method was technically simpler and more rapid. The TaqMan assay also allows for high-throughput genotyping, making it ideal for epidemiologic study of the live attenuated varicella vaccine.
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Registered trials
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