ArticleProceedings of the National Academy of Sciences of the United States of America2004
A Ca2+/calmodulin-dependent protein kinase required for symbiotic nodule development: Gene identification by transcript-based cloning.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 149 papers.
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149 citing papers in PubMed, 482 citations in OpenAlex.
- ESN3, a nodule-specific small peptide essential for symbiotic nitrogen fixation in Medicago truncatula.The New phytologist · 2026Article
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- A Phytosulfokine signaling module activates the Nod factor receptor to control soybean nodulation.Nature communications · 2026Article
- Molecular Insights into the Positive Role of Soybean Nodulation by GmWRKY17.International journal of molecular sciences · 2025Article
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- Transcriptomic insights into mycorrhizal interactions with tomato root: a comparative study of short- and long-term post-inoculation responses.Frontiers in genetics · 2024Article
- GmBES1-1 dampens the activity of GmNSP1/2 to mediate brassinosteroid inhibition of nodulation in soybean.Plant communications · 2023Article
- Genome-Wide Identification of the CDPK Gene Family and Their Involvement in Taproot Cracking in Radish.International journal of molecular sciences · 2023Article
- Biological and genomic analysis of a symbiotic nitrogen fixation defective mutant inFrontiers in plant science · 2023Article
- The calcium connection: exploring the intricacies of calcium signaling in plant-microbe interactions.Frontiers in plant science · 2023Review
- Progress in the Self-Regulation System in Legume Nodule Development-AON (Autoregulation of Nodulation).International journal of molecular sciences · 2022Review
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- Salt Stress Enhances Early Symbiotic Gene Expression inMolecular plant-microbe interactions : MPMI · 2021Article
- MycoRed: Betalain pigments enable in vivo real-time visualisation of arbuscular mycorrhizal colonisation.PLoS biology · 2021Article
- Perception of lipo-chitooligosaccharides by the bioenergy cropPlant signaling & behavior · 2021Review
- VAPYRIN attenuates defence by repressing PR gene induction and localized lignin accumulation during arbuscular mycorrhizal symbiosis of Petunia hybrida.The New phytologist · 2021Article
- Dephosphorylation of LjMPK6 by Phosphatase LjPP2C is Involved in Regulating Nodule Organogenesis inInternational journal of molecular sciences · 2020Article
89 more citing papers are in PubMed but not listed here.
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In the establishment of the legume-rhizobial symbiosis, bacterial lipochitooligosaccharide signaling molecules termed Nod factors activate the formation of a novel root organ, the nodule. Nod factors elicit several responses in plant root hair cells, including oscillations in cytoplasmic calcium levels (termed calcium spiking) and alterations in root hair growth. A number of plant mutants with defects in the Nod factor signaling pathway have been identified. One such Medicago truncatula mutant, dmi3, exhibits calcium spiking and root hair swelling in response to Nod factor, but fails to initiate symbiotic gene expression or cell divisions for nodule formation. On the basis of these data, it is thought that the dmi3 mutant perceives Nod factor but fails to transduce the signal downstream of calcium spiking. Additionally, the dmi3 mutant is defective in the symbiosis with mycorrhizal fungi, indicating the importance of the encoded protein in multiple symbioses. We report the identification of the DMI3 gene, using a gene cloning method based on transcript abundance. We show that transcript-based cloning is a valid approach for cloning genes in barley, indicating the value of this technology in crop plants. DMI3 encodes a calcium/calmodulin-dependent protein kinase. Mutants in pea sym9 have phenotypes similar to dmi3 and have alterations in this gene. The DMI3 class of proteins is well conserved among plants that interact with mycorrhizal fungi, but it is less conserved in Arabidopsis thaliana, which does not participate in the mycorrhizal symbiosis.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.