ArticleProceedings of the National Academy of Sciences of the United States of America2004
A regulator that inhibits transcription by targeting an intersubunit interaction of the RNA polymerase holoenzyme.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed.
- Aromatic patch in whiB-like transcription factors facilitates primary sigma factor interaction in mycobacterium tuberculosis.Communications biology · 2026Article
- Phage protein Gp11 blocksmBio · 2024Article
- Redefining fundamental concepts of transcription initiation in bacteria.Nature reviews. Genetics · 2020Review
- Structural basis of non-canonical transcriptional regulation by the σA-bound iron-sulfur protein WhiB1 in M. tuberculosis.Nucleic acids research · 2020Article
- Structural basis of σ appropriation.Nucleic acids research · 2019Article
- A novel RNA polymerase-binding protein that interacts with a sigma-factor docking site.Molecular microbiology · 2017Article
- Local and global regulation of transcription initiation in bacteria.Nature reviews. Microbiology · 2016Review
- Visualizing the phage T4 activated transcription complex of DNA and E. coli RNA polymerase.Nucleic acids research · 2016Article
- Bacteriophage T4 MotA activator and the β-flap tip of RNA polymerase target the same set of σ70 carboxyl-terminal residues.The Journal of biological chemistry · 2011Article
- Genetic evidence for a novel interaction between transcriptional activator SoxS and region 4 of the σ(70) subunit of RNA polymerase at class II SoxS-dependent promoters in Escherichia coli.Journal of molecular biology · 2011Article
- Structural basis for the bacterial transcription-repair coupling factor/RNA polymerase interaction.Nucleic acids research · 2010Article
- A mutation within the β subunit of Escherichia coli RNA polymerase impairs transcription from bacteriophage T4 middle promoters.Journal of bacteriology · 2010Article
- Transcriptional control in the prereplicative phase of T4 development.Virology journal · 2010Review
- Determinants of affinity and activity of the anti-sigma factor AsiA.Biochemistry · 2010Article
- Utilization of variably spaced promoter-like elements by the bacterial RNA polymerase holoenzyme during early elongation.Molecular microbiology · 2010Article
- Molecular evolution of multisubunit RNA polymerases: structural analysis.Journal of molecular biology · 2010Article
- Direct activator/co-activator interaction is essential for bacteriophage T4 middle gene expression.Molecular microbiology · 2009Article
- A regulator from Chlamydia trachomatis modulates the activity of RNA polymerase through direct interaction with the beta subunit and the primary sigma subunit.Genes & development · 2009Article
- The bacteriophage T4 AsiA protein contacts the beta-flap domain of RNA polymerase.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Mutagenesis of region 4 of sigma 28 from Chlamydia trachomatis defines determinants for protein-protein and protein-DNA interactions.Journal of bacteriology · 2009Article
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10 authors.
Funding
Abstract
The structures of the bacterial RNA polymerase holoenzyme have provided detailed information about the intersubunit interactions within the holoenzyme. Functional analysis indicates that one of these is critical in enabling the holoenzyme to recognize the major class of bacterial promoters. It has been suggested that this interaction, involving the flap domain of the beta subunit and conserved region 4 of the sigma subunit, is a potential target for regulation. Here we provide genetic and biochemical evidence that the sigma region 4/beta-flap interaction is targeted by the transcription factor AsiA. Specifically, we show that AsiA competes directly with the beta-flap for binding to sigma region 4, thereby inhibiting transcription initiation by disrupting the sigma region 4/beta-flap interaction.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.