ArticleProceedings of the National Academy of Sciences of the United States of America2004
Regulation of cyclin-dependent kinase activity during mitotic exit and maintenance of genome stability by p21, p27, and p107.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 35 citations in OpenAlex.
- Increased Expression of NPM1 Suppresses p27Cancers · 2020Article
- MASTL: A novel therapeutic target for Cancer Malignancy.Cancer medicine · 2020Review
- Aberrant activation of cyclin A-CDK induces G2/M-phase checkpoint in human cells.Cell cycle (Georgetown, Tex.) · 2020Article
- Article
- pRb-E2F signaling in life of mesenchymal stem cells: Cell cycle, cell fate, and cell differentiation.Genes & diseases · 2014Review
- Cyclin E2 induces genomic instability by mechanisms distinct from cyclin E1.Cell cycle (Georgetown, Tex.) · 2013Article
- Article
- Clinical significance of miR-221 and its inverse correlation with p27Kip¹ in hepatocellular carcinoma.Molecular biology reports · 2011Article
- Cyclin A promotes S-phase entry via interaction with the replication licensing factor Mcm7.Molecular and cellular biology · 2011Article
- Simian virus 40 activates ATR-Delta p53 signaling to override cell cycle and DNA replication control.Journal of virology · 2010Article
- Article
- p27kip1 deficiency impairs G2/M arrest in response to DNA damage, leading to an increase in genetic instability.Molecular and cellular biology · 2008Article
- Cooperation between p27 and p107 during endochondral ossification suggests a genetic pathway controlled by p27 and p130.Molecular and cellular biology · 2007Article
- Regulation of late G1/S phase transition and APC Cdh1 by reactive oxygen species.Molecular and cellular biology · 2006Article
- Novel functions of plant cyclin-dependent kinase inhibitors, ICK1/KRP1, can act non-cell-autonomously and inhibit entry into mitosis.The Plant cell · 2005Article
- Pocket proteins and cell cycle control.Oncogene · 2005Review
- Tissue microarray for high-throughput analysis of gene expression profiles in hepatocellular carcinoma.World journal of gastroenterology · 2005Article
- Evidence for a p27 tumor suppressive function independent of its role regulating cell proliferation in the prostate.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To study the regulation of cyclin-dependent kinase (CDK) activity during mitotic exit in mammalian cells, we constructed murine cell lines that constitutively express a stabilized mutant of cyclin A (cyclin A47). Even though cyclin A47 was expressed throughout mitosis and in G1 cells, its associated CDK activity was inactivated after the transition from metaphase to anaphase. Cyclin A47 associated with both p21 and p27 during mitotic exit, implicating these proteins in CDK inactivation. However, cyclin A47 was fully inhibited during the M-to-G1 transition in p21(-/-) p27(-/-) fibroblasts. Also, the CDKs associated with cyclin A47 were not inactivated by phosphorylation at tyrosines. The protein responsible for CDK inactivation during mitotic exit in p21/p27 null cells was the Rb family member, p107. p107 bound to cyclin A47 when p21 and p27 were absent, and cyclin A47-CDK activity was not inactivated during the M-to-G1 transition in p21(-/-) p27(-/-) p107(-/-) null fibroblasts. Enforced expression of cyclin A in cells lacking all three CDK inhibitors induced rapid tetraploidization, indicative of mitotic failure/endoreduplication. We concluded that cyclin proteolysis and CDK inhibitors constitute redundant pathways that control cyclin A-CDK activity during mitotic exit in mammalian cells and that loss of these pathways can cause genetic instability.
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