ArticleTransgenic research2004
Retention of the developmental pluripotency in medaka embryonic stem cells after gene transfer and long-term drug selection for gene targeting in fish.
Article in Transgenic research, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 61 citations in OpenAlex.
- Establishment and longitudinal characterisation of a feeder-free embryonic stem-like cell line (ATES1) derived from blastomeres of the climbing perch, Anabas testudineus.Fish physiology and biochemistry · 2026Article
- Establishment of aBiology · 2022Article
- Establishment, characterization, and transfection potential of a new continuous fish cell line (CAM) derived from the muscle tissue of grass goldfish (Carassius auratus).In vitro cellular & developmental biology. Animal · 2021Article
- Screening for Antiviral Medaka Haploid Embryonic Stem Cells by Genome Wide Mutagenesis.Marine biotechnology (New York, N.Y.) · 2019Article
- Gene delivery into Siberian sturgeon cell lines by commercial transfection reagents.In vitro cellular & developmental biology. Animal · 2019Article
- Subcellular redistribution and sequential recruitment of macromolecular components during SGIV assembly.Protein & cell · 2016Article
- Medaka Oct4 is essential for pluripotency in blastula formation and ES cell derivation.Stem cell reviews and reports · 2015Article
- Gene transfer and genome-wide insertional mutagenesis by retroviral transduction in fish stem cells.PloS one · 2015Article
- Article
- Nanos3 gene targeting in medaka ES cells.International journal of biological sciences · 2013Article
- Differential evolution of duplicated medakafish mitf genes.International journal of biological sciences · 2013Article
- Interordinal chimera formation between medaka and zebrafish for analyzing stem cell differentiation.Stem cells and development · 2012Article
- Fish stem cell cultures.International journal of biological sciences · 2011Review
- Development and partial characterization of new marine cell line from brain of Asian sea bass Lates calcarifer for virus isolation.In vitro cellular & developmental biology. Animal · 2011Article
- Development and characterization of a new marine fish cell line from turbot (Scophthalmus maximus).Fish physiology and biochemistry · 2010Article
- Accessibility of host cell lineages to medaka stem cells depends on genetic background and irradiation of recipient embryos.Cellular and molecular life sciences : CMLS · 2010Article
- Efficient transfection of primary zebrafish fibroblasts by nucleofection.Cytotechnology · 2006Article
- Homologous recombination in zebrafish ES cells.Transgenic research · 2006Article
- Establishment of a normal medakafish spermatogonial cell line capable of sperm production in vitro.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- Development and characterization of two new cell lines from milkfish (Chanos chanos) and grouper (Epinephelus coioides) for virus isolation.Marine biotechnology (New York, N.Y.)Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Embryonic stem (ES) cells provide a unique tool for introducing random or targeted genetic alterations, because it is possible that the desired, but extremely rare recombinant genotypes can be screened by drug selection. ES cell-mediated transgenesis has so far been limited to the mouse. In the fish medaka (Oryzias latipes) several ES cell lines have been made available. Here we report the optimized conditions for gene transfer and drug selection in the medaka ES cell line MES1 as a prelude for gene targeting in fish. MES1 cells gave rise to a moderate to high transfection efficiency by the calcium phosphate co-precipitation (5%), commercial reagents Fugene (11%), GeneJuice (21%) and electroporation (>30%). Transient gene transfer and CAT reporter assay revealed that several enhancers/promoters and their combinations including CMV, RSV and ST (the SV40 virus early gene enhancer linked to the thymidine kinase promoter) were suitable regulatory sequences to drive transgene expression in the MES1 cells. We show that neo, hyg or pac conferred resistance to G418, hygromycin or puromycin for positive selection, while the HSV-tk generated sensitivity to ganciclovir for negative selection. The positive-negative selection procedure that is widely used for gene targeting in mouse ES cells was found to be effective also in MES1 cells. Importantly, we demonstrate that MES1 cells after gene transfer and long-term drug selection retained the developmental pluripotency, as they were able to undergo induced differentiation in vitro and to contribute to various tissues and organs during chimeric embryogenesis.
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