ArticleMolecular diagnosis : a journal devoted to the understanding of human disease through the clinical application of molecular biology2003
PCR-based detection of a cystic fibrosis epidemic strain of Pseudomonas Aeruginosa.
Article in Molecular diagnosis : a journal devoted to the understanding of human disease through the clinical application of molecular biology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 23 citations in OpenAlex.
- Evolutionary loss of an antibiotic efflux pump increases Pseudomonas aeruginosa quorum sensing mediated virulence in vivo.Nature communications · 2025Article
- Virulence Mechanisms ofFrontiers in microbiology · 2022Review
- Epidemiology, Biology, and Impact of Clonal Pseudomonas aeruginosa Infections in Cystic Fibrosis.Clinical microbiology reviews · 2018Review
- Observational
- Sub-inhibitory concentrations of some antibiotics can drive diversification of Pseudomonas aeruginosa populations in artificial sputum medium.BMC microbiology · 2013Article
- Halting the spread of epidemic pseudomonas aeruginosa in an adult cystic fibrosis centre: a prospective cohort study.JRSM short reports · 2013Article
- Differential infection properties of three inducible prophages from an epidemic strain of Pseudomonas aeruginosa.BMC microbiology · 2012Article
- A diagnostic PCR assay for the detection of an Australian epidemic strain of Pseudomonas aeruginosa.Annals of clinical microbiology and antimicrobials · 2010Article
- Impact of Pseudomonas aeruginosa genomic instability on the application of typing methods for chronic cystic fibrosis infections.Journal of clinical microbiology · 2010Article
- Translational genomics to develop a Salmonella enterica serovar Paratyphi A multiplex polymerase chain reaction assay.The Journal of molecular diagnostics : JMD · 2007Article
- Widespread pyocyanin over-production among isolates of a cystic fibrosis epidemic strain.BMC microbiology · 2007Article
- A cystic fibrosis epidemic strain of Pseudomonas aeruginosa displays enhanced virulence and antimicrobial resistance.Journal of bacteriology · 2005Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe Liverpool epidemic strain (LES) of Pseudomonas aeruginosa is widespread among patients with cystic fibrosis (CF) in specialist centers around Liverpool and elsewhere in the UK. This study evaluates a new diagnostic PCR assay based on a unique DNA sequence (PS21) of LES, for its identification of colonies directly from sputum.
methodsOne hundred and fifty-eight sputum samples from 92 patients were cultured and P. aeruginosa isolates were typed by PS21 PCR and pulsed-field gel electrophoresis (PFGE). Subsequently, PS21 PCR was performed directly on sputum and the results were compared with culture, PFGE, and PS21 PCR typing.
resultsEighty patients were colonized with P. aeruginosa, 63 by LES (79%). There was 100% concordance between PS21 PCR on colonies and PFGE typing. The sensitivity and specificity of PS21 PCR directly on sputum was 98.2% and 93.6%, respectively.
conclusionsThis study shows that PS21 PCR can be used for simple and rapid screening of LES colonization in CF patients.
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