ArticleThe Journal of experimental medicine2004
T cell receptor engagement leads to phosphorylation of clathrin heavy chain during receptor internalization.
Article in The Journal of experimental medicine, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed.
- TCRζ-driven pre-signaling organization of Lck in Rab11⁺ endosomes shapes TCR activation.Cellular & molecular biology letters · 2026Article
- XAF1 is secreted from stressed tumor cells to activate T cell-mediated tumor surveillance via Lck-ERK signaling.Neoplasia (New York, N.Y.) · 2025Article
- Preclinical Immunogenicity and Efficacy Studies for Therapeutic Vaccines for Human Papillomavirus-Type-16-Associated Cancer.Vaccines · 2024Article
- Trogocytic molting of T cell microvilli upregulates T cell receptor surface expression and promotes clonal expansion.Nature communications · 2023Article
- Clathrin mediates both internalization and vesicular release of triggered T cell receptor at the immunological synapse.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Mechanisms and functions of endocytosis in T cells.Cell communication and signaling : CCS · 2021Review
- F-Actin Dynamics in the Regulation of Endosomal Recycling and Immune Synapse Assembly.Frontiers in cell and developmental biology · 2021Review
- Human FCHO1 deficiency reveals role for clathrin-mediated endocytosis in development and function of T cells.Nature communications · 2020Article
- Orchestration of Immunological Synapse Assembly by Vesicular Trafficking.Frontiers in cell and developmental biology · 2019Review
- Polarity of CD4+ T cells towards the antigen presenting cell is regulated by the Lck adapter TSAd.Scientific reports · 2018Article
- The Neutral Sphingomyelinase 2 Is Required to Polarize and Sustain T Cell Receptor Signaling.Frontiers in immunology · 2018Article
- Arf6 and Rab22 mediate T cell conjugate formation by regulating clathrin-independent endosomal membrane trafficking.Journal of cell science · 2017Article
- A missense mutation in TFRC, encoding transferrin receptor 1, causes combined immunodeficiency.Nature genetics · 2016Article
- Orchestrating Lymphocyte Polarity in Cognate Immune Cell-Cell Interactions.International review of cell and molecular biology · 2016Review
- Vesicular Trafficking to the Immune Synapse: How to Assemble Receptor-Tailored Pathways from a Basic Building Set.Frontiers in immunology · 2016Review
- Unconventional functions for clathrin, ESCRTs, and other endocytic regulators in the cytoskeleton, cell cycle, nucleus, and beyond: links to human disease.Cold Spring Harbor perspectives in biology · 2014Review
- The adaptor protein GULP promotes Jedi-1-mediated phagocytosis through a clathrin-dependent mechanism.Molecular biology of the cell · 2014Article
- Allogeneic T cell responses are regulated by a specific miRNA-mRNA network.The Journal of clinical investigation · 2013Article
- The human CD8β M-4 isoform dominant in effector memory T cells has distinct cytoplasmic motifs that confer unique properties.PloS one · 2013Article
- Transient combination therapy targeting the immune synapse abrogates T cell responses and prolongs allograft survival in mice.PloS one · 2013Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
T cell receptor (TCR) internalization by clathrin-coated vesicles after encounter with antigen has been implicated in the regulation of T cell responses. We demonstrate that TCR internalization after receptor engagement and TCR signaling involves inducible phosphorylation of clathrin heavy chain (CHC) in both CD4+ and CD8+ human T cells. Studies with mutant Jurkat T cells implicate the Src family kinase Lck as the responsible enzyme and its activity in this process is influenced by the functional integrity of the downstream signaling molecule ZAP-70. CHC phosphorylation positively correlates with ligand-induced TCR internalization in both CD4+ and CD8+ T cells, and CHC phosphorylation as a result of basal Lck activity is also implicated in constitutive TCR endocytosis by CD4+ T cells. Remarkably, irreversible CHC phosphorylation in the presence of pervanadate reduced both constitutive and ligand-induced TCR internalization in CD4+ T cells, and immunofluorescence studies revealed that this inhibition affected the early stages of TCR endocytosis from the plasma membrane. Thus, we propose that CHC phosphorylation and dephosphorylation are involved in TCR internalization and that this is a regulatory mechanism linking TCR signaling to endocytosis.
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