ArticleThe Journal of cell biology2004
Pheromone-induced polarization is dependent on the Fus3p MAPK acting through the formin Bni1p.
Article in The Journal of cell biology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 54 papers.
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Who cites it
54 citing papers in PubMed, 115 citations in OpenAlex.
- Constitutively active RAS prolongs Cdc42 signalling, while MAPK signalling is attenuated during fission yeast mating.PLoS genetics · 2026Article
- The macroevolution of filamentation morphology across the Saccharomycotina yeast subphylum.FEMS yeast research · 2026Article
- The Macroevolution of Filamentation Morphology Across thebioRxiv : the preprint server for biology · 2025Article
- Role of Gα-MAPK interaction in mating ofMolecular biology of the cell · 2025Article
- Functional redundancy and formin-isoform independent localization of tropomyosin paralogs in Saccharomyces cerevisiae.PLoS genetics · 2025Article
- The roles of yeast formins and their regulators Bud6 and Bil2 in the pheromone response.Molecular biology of the cell · 2024Article
- Global quantitative understanding of non-equilibrium cell fate decision-making in response to pheromone.iScience · 2023Article
- Quantifying nonequilibrium dynamics and thermodynamics of cell fate decision making in yeast under pheromone induction.Biophysics reviews · 2023Article
- Phosphorylation of RGS regulates MAP kinase localization and promotes completion of cytokinesis.Life science alliance · 2022Article
- Orientation of Cell Polarity by Chemical Gradients.Annual review of biophysics · 2022Review
- Cla4p Kinase Activity Is Down-Regulated by Fus3p during Yeast Mating.Biomolecules · 2022Article
- Correlation Between Improved Mating Efficiency and Weakened Scaffold-Kinase Interaction in the Mating Pheromone Response Pathway Revealed by Interspecies Complementation.Frontiers in microbiology · 2022Article
- Article
- Negative control of cytokinesis by stress-activated MAPK signaling.Current genetics · 2021Review
- Stress-Activated Protein Kinase Signalling Regulates Mycoparasitic Hyphal-Hyphal Interactions inJournal of fungi (Basel, Switzerland) · 2021Article
- Review
- Mitotic and pheromone-specific intrinsic polarization cues interfere with gradient sensing inProceedings of the National Academy of Sciences of the United States of America · 2020Article
- Spatio-temporal MAPK dynamics mediate cell behavior coordination during fungal somatic cell fusion.Journal of cell science · 2018Article
- Calcineurin, the CaMolecular biology of the cell · 2017Article
- Spatial and temporal signal processing and decision making by MAPK pathways.The Journal of cell biology · 2017Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
During mating, budding yeast cells reorient growth toward the highest concentration of pheromone. Bni1p, a formin homologue, is required for this polarized growth by facilitating cortical actin cable assembly. Fus3p, a pheromone-activated MAP kinase, is required for pheromone signaling and cell fusion. We show that Fus3p phosphorylates Bni1p in vitro, and phosphorylation of Bni1p in vivo during the pheromone response is dependent on Fus3p. fus3 mutants exhibited multiple phenotypes similar to bni1 mutants, including defects in actin and cell polarization, as well as Kar9p and cytoplasmic microtubule localization. Disruption of the interaction between Fus3p and the receptor-associated Galpha subunit caused similar mutant phenotypes. After pheromone treatment, Bni1p-GFP and Spa2p failed to localize to the cortex of fus3 mutants, and cell wall growth became completely unpolarized. Bni1p overexpression suppressed the actin assembly, cell polarization, and cell fusion defects. These data suggest a model wherein activated Fus3p is recruited back to the cortex, where it activates Bni1p to promote polarization and cell fusion.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.