ArticleApplied and environmental microbiology2004
GC fractionation enhances microbial community diversity assessment and detection of minority populations of bacteria by denaturing gradient gel electrophoresis.
Article in Applied and environmental microbiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed.
- Strengths and weaknesses in techniques employed to measure the effects of dietary modulation on intestinal microbiota composition and function.Animal nutrition (Zhongguo xu mu shou yi xue hui) · 2026Review
- Genomic recovery from rare terrestrial microbes enabled by DNA-based GC-fractionation.ISME communications · 2025Article
- Study ofMicroorganisms · 2024Article
- Wildlife fecal microbiota exhibit community stability across a longitudinal semi-controlled non-invasive sampling experiment.Frontiers in microbiomes · 2024Article
- Soil Microbial Community Involved in Nitrogen Cycling in Rice Fields Treated with Antiozonant under Ambient Ozone.Applied and environmental microbiology · 2023Article
- Non-invasive monitoring of multiple wildlife health factors by fecal microbiome analysis.Ecology and evolution · 2022Article
- Review
- Influential factors on the composition of the conventionally raised broiler gastrointestinal microbiomes.Poultry science · 2020Review
- Article
- The impact of Bacillus subtilis DSM 32315 on the pathology, performance, and intestinal microbiome of broiler chickens in a necrotic enteritis challenge.Poultry science · 2019Article
- Cecal microbiota of Tibetan Chickens from five geographic regions were determined by 16S rRNA sequencing.MicrobiologyOpen · 2016Article
- Emerging strategies and integrated systems microbiology technologies for biodiscovery of marine bioactive compounds.Marine drugs · 2014Review
- Isolation and characterization of an agaro-oligosaccharide (AO)-hydrolyzing bacterium from the gut microflora of Chinese individuals.PloS one · 2014Article
- Metaproteomics analysis reveals the adaptation process for the chicken gut microbiota.Applied and environmental microbiology · 2014Article
- Higher-level production of volatile fatty acids in vitro by chicken gut microbiotas than by human gut microbiotas as determined by functional analyses.Applied and environmental microbiology · 2012Article
- Effect of high contents of dietary animal-derived protein or carbohydrates on canine faecal microbiota.BMC veterinary research · 2012Article
- (±)-catechin, a root exudate of the invasive centaurea stoebe lam. (Spotted knapweed) exhibits bacteriostatic activity against multiple soil bacterial populations.Journal of chemical ecology · 2011Article
- Worldwide prevalence of class 2 integrases outside the clinical setting is associated with human impact.Applied and environmental microbiology · 2009Article
- Sequence analysis of percent G+C fraction libraries of human faecal bacterial DNA reveals a high number of Actinobacteria.BMC microbiology · 2009Article
- Extensive phylogenetic analysis of a soil bacterial community illustrates extreme taxon evenness and the effects of amplicon length, degree of coverage, and DNA fractionation on classification and ecological parameters.Applied and environmental microbiology · 2009Article
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4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Effectively and accurately assessing total microbial community diversity is one of the primary challenges in modern microbial ecology. This is particularly true with regard to the detection and characterization of unculturable populations and those present only in low abundance. We report a novel strategy, GC fractionation combined with denaturing gradient gel electrophoresis (GC-DGGE), which combines mechanistically different community analysis approaches to enhance assessment of microbial community diversity and detection of minority populations of microbes. This approach employs GC fractionation as an initial step to reduce the complexity of the community in each fraction. This reduced complexity facilitates subsequent detection of diversity in individual fractions. DGGE analysis of individual fractions revealed bands that were undetected or only poorly represented when total bacterial community DNA was analyzed. Also, directed cloning and sequencing of individual bands from DGGE lanes corresponding to individual G+C fractions allowed detection of numerous phylotypes that were not recovered using a traditional random cloning and sequencing approach.
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