ArticleApplied and environmental microbiology2004
Use of PCR-restriction fragment length polymorphism of inlA for rapid screening of Listeria monocytogenes strains deficient in the ability to invade Caco-2 cells.
Article in Applied and environmental microbiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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39 citing papers in PubMed.
- Molecular Characterization of Virulence-Related Genes in Listeria monocytogenes Isolated from Retail Meats in Sapporo, Japan.Current microbiology · 2025Article
- Current methodologies available to evaluate the virulence potential amongFrontiers in microbiology · 2024Review
- Evaluation of the Virulence Potential ofInternational journal of molecular sciences · 2023Article
- A Comprehensive Virulence and Resistance Characteristics ofInternational journal of molecular sciences · 2023Article
- Genomic Surveillance of Listeria monocytogenes in Taiwan, 2014 to 2019.Microbiology spectrum · 2022Article
- Review
- Different Shades ofFrontiers in microbiology · 2021Article
- Article
- Whole-genome sequencing reveals Listeria monocytogenes diversity and allows identification of long-term persistent strains in Brazil.Environmental microbiology · 2019Article
- Exploring Listeria monocytogenes Transcriptomes in Correlation with Divergence of Lineages and Virulence as Measured in Galleria mellonella.Applied and environmental microbiology · 2019Article
- Isolation and characterization ofIranian journal of microbiology · 2019Article
- Article
- Genomic Diversity ofGenes · 2018Article
- Occurrence, Antibiotic Resistance, and Population Diversity ofFrontiers in microbiology · 2018Article
- Genotypes Associated withFrontiers in microbiology · 2017Article
- Tolerance of Listeria monocytogenes to Quaternary Ammonium Sanitizers Is Mediated by a Novel Efflux Pump Encoded by emrE.Applied and environmental microbiology · 2016Article
- Prevalence and distribution of Listeria monocytogenes inlA alleles prone to phase variation and inlA alleles with premature stop codon mutations among human, food, animal, and environmental isolates.Applied and environmental microbiology · 2015Article
- Reservoirs of listeria species in three environmental ecosystems.Applied and environmental microbiology · 2014Article
- Examination of food chain-derived Listeria monocytogenes strains of different serotypes reveals considerable diversity in inlA genotypes, mutability, and adaptation to cold temperatures.Applied and environmental microbiology · 2013Article
- Evidence of autoinduction heterogeneity via expression of the Agr system of Listeria monocytogenes at the single-cell level.Applied and environmental microbiology · 2011Article
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5 authors.
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Abstract
A PCR-restriction fragment length polymorphism (RFLP) method was developed in order to screen a large number of strains for impaired adhesion to epithelial cells due to expression of truncated InlA. inlA polymorphism was analyzed by PCR-RFLP in order to correlate inlA PCR-RFLP profiles and production of truncated InlA. Thirty-seven Listeria monocytogenes strains isolated from various sources, including five noninvasive and two invasive reference strains, were screened. Two endonucleases (AluI and Tsp509I) were used, and they generated five composite profiles. Thirteen L. monocytogenes isolates were characterized by two specific PCR-RFLP profiles similar to PCR-RFLP profiles of noninvasive reference strains previously described as strains that produce truncated InlA. Ten of the 13 isolates showed low abilities to invade human epithelial Caco-2 cells. However, 4 of the 13 isolates were able to invade Caco-2 cells like reference strains containing complete InlA. Sequencing of inlA and Western blot analysis confirmed that truncated InlA was expressed in the 10 L. monocytogenes strains which were isolated from food. This PCR-RFLP method allowed us to identify 10 new strains expressing a truncated internalin. Based on the results obtained in this study, the PCR-RFLP method seems to be an interesting method for rapidly screening L. monocytogenes strains deficient in the ability to invade Caco-2 cells when a sizeable number of strains are studied.
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