ArticlePlanta2004
Xylem sap protein composition is conserved among different plant species.
Article in Planta, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.
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The trial behind it
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Who cites it
52 citing papers in PubMed, 172 citations in OpenAlex.
- Untargeted Metabolomics of Xylem Sap Exudates in Two Common Bean Genotypes with Contrasting Growth Rates Under Water Deficit During Pod Filling.Metabolites · 2026Article
- Xylem Sap Proteome Analysis Provides Insight into Root-Shoot Communication in Response to flg22.Plants (Basel, Switzerland) · 2024Article
- Review
- Symbioses shape feeding niches and diversification across insects.Nature ecology & evolution · 2023Article
- Lipid-Coated Nanobubbles in Plants.Nanomaterials (Basel, Switzerland) · 2023Review
- Insights into the Methodological, Biotic and Abiotic Factors Influencing the Characterization of Xylem-Inhabiting Microbial Communities of Olive Trees.Plants (Basel, Switzerland) · 2023Review
- Collection of Xylem Exudates from the Model PlantBio-protocol · 2022Article
- Identification of Diverse Stress-Responsive Xylem Sap Peptides in Soybean.International journal of molecular sciences · 2022Article
- Characterization of Oligopeptides inLife (Basel, Switzerland) · 2022Article
- Insights into the mechanism of Huanglongbing tolerance in the Australian finger lime (Frontiers in plant science · 2022Article
- Physiology and molecular biology of plants : an international journal of functional plant biology · 2021Article
- Preservative potential of biosynthesized silver nanoparticles: prevention of xylem occlusion and microbial proliferation at postharvest stage of preservation.Environmental science and pollution research international · 2021Article
- Appropriate NHFrontiers in plant science · 2021Article
- Comparative Proteomic Analysis ofMolecules (Basel, Switzerland) · 2020Article
- An efficient low-cost xylem sap isolation method for bacterial wilt assays in tomato.Applications in plant sciences · 2020Article
- Role of Pea LTPs and Abscisic Acid in Salt-Stressed Roots.Biomolecules · 2019Article
- Integrated metatranscriptome and transcriptome reveals the microbial community composition and physiological function of xylem sap on grapevine during bleeding period.Genes & genomics · 2019Article
- Plant Leucine-Rich Repeat Receptor Kinase (LRR-RK): Structure, Ligand Perception, and Activation Mechanism.Molecules (Basel, Switzerland) · 2019Review
- Proteomic changes in the xylem sap of Brassica napus under cadmium stress and functional validation.BMC plant biology · 2019Article
- Trans-Graft Protection Against Pierce's Disease Mediated by Transgenic Grapevine Rootstocks.Frontiers in plant science · 2019Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Xylem sap from broccoli (Brassica oleracea L. cv. Calabrais), rape (Brassica napus L. cv. Drakkar), pumpkin (Cucurbita maxima Duch. cv. gelber Zentner) and cucumber (Cucumis sativus L. cv. Hoffmanns Giganta) was collected by root pressure exudation from the surface of cut stems of healthy, adult plants. Total protein concentrations were in the range of 100 microg ml(-1). One-dimensional gel electrophoresis (SDS-PAGE) resulted in 10-20 visible protein bands in a molecular mass range from 10 to 100 kDa. The main bands were cut out, digested with trypsin, and analysed using tandem mass spectrometry. Fifty bands resulted in amino acid sequence information that was used to perform database similarity searches. Sequences from 30 bands showed high homology to proteins present in databases. Among them, we found mostly peroxidases, but could also identify the lectin-like xylem protein XSP30, a glycine-rich protein, serine proteases, an aspartyl protease family protein, chitinases, and a lipid transfer protein-like polypeptide. Sequence analysis predicted apoplastic secretion signals for all database entries similar to the partial xylem protein sequences. This and the lack of cross-reactivity with phloem protein-specific antibodies suggest that the proteins really originate from the xylem and do not result from phloem contamination. Most of the highly similar proteins probably function in repair and defence reactions. Some of the most abundant proteins (peroxidases, chitinases, serine proteases) were present in xylem exudate of all species analysed, often in more than one band. This indicates an important basic role of these proteins in maintaining xylem function.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.