ArticlePlant physiology2004
Identification and characterization of respirasomes in potato mitochondria.
Article in Plant physiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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41 citing papers in PubMed, 166 citations in OpenAlex.
- Plant supercomplex I + III2 structure and function: implications for the growing field.Biochemical Society transactions · 2024Review
- OXPHOS Organization and Activity in Mitochondria of Plants with Different Life Strategies.International journal of molecular sciences · 2023Article
- The biogenesis and regulation of the plant oxidative phosphorylation system.Plant physiology · 2023Review
- Plant-specific features of respiratory supercomplex I + IIINature plants · 2023Article
- Atomic structures of respiratory complex IIIeLife · 2021Article
- Carbon and Nitrogen Sources Have No Impact on the Organization and Composition ofJournal of fungi (Basel, Switzerland) · 2021Article
- Specific features and assembly of the plant mitochondrial complex I revealed by cryo-EM.Nature communications · 2020Article
- Article
- Use of in-gel peroxidase assay for cytochromeBiology open · 2020Article
- Mutations inCells · 2019Article
- The role of cardiolipin concentration and acyl chain composition on mitochondrial inner membrane molecular organization and function.Biochimica et biophysica acta. Molecular and cell biology of lipids · 2019Review
- CytochromeComputational and structural biotechnology journal · 2019Review
- Conserved in situ arrangement of complex I and IIIProceedings of the National Academy of Sciences of the United States of America · 2018Article
- Cardiolipin Supports Respiratory Enzymes in Plants in Different Ways.Frontiers in plant science · 2017Article
- Molecular mechanism of cardiolipin-mediated assembly of respiratory chain supercomplexes.Chemical science · 2016Article
- Supercomplex-associated Cox26 protein binds to cytochrome c oxidase.Biochimica et biophysica acta · 2016Article
- Combined defects in oxidative phosphorylation and fatty acid β-oxidation in mitochondrial disease.Bioscience reports · 2016Review
- Mitochondrial Stat3, the Need for Design Thinking.International journal of biological sciences · 2016Review
- The Arabidopsis COX11 Homolog is Essential for Cytochrome c Oxidase Activity.Frontiers in plant science · 2015Article
- The importance of cardiolipin synthase for mitochondrial ultrastructure, respiratory function, plant development, and stress responses in Arabidopsis.The Plant cell · 2013Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Plant mitochondria were previously shown to comprise respiratory supercomplexes containing cytochrome c reductase (complex III) and NADH dehydrogenase (complex I) of I(1)III(2) and I(2)III(4) composition. Here we report the discovery of additional supercomplexes in potato (Solanum tuberosum) mitochondria, which are of lower abundance and include cytochrome c oxidase (complex IV). Highly active mitochondria were isolated from potato tubers and stems, solubilized by digitonin, and subsequently analyzed by Blue-native (BN) polyacrylamide gel electrophoresis (PAGE). Visualization of supercomplexes by in-gel activity stains for complex IV revealed five novel supercomplexes of 850, 1,200, 1,850, 2,200, and 3,000 kD in potato tuber mitochondria. These supercomplexes have III(2)IV(1), III(2)IV(2), I(1)III(2)IV(1), I(1)III(2)IV(2), and I(1)III(2)IV(4) compositions as shown by two-dimensional BN/sodium dodecyl sulfate (SDS)-PAGE and BN/BN-PAGE in combination with activity stains for cytochrome c oxidase. Potato stem mitochondria include similar supercomplexes, but complex IV is partially present in a smaller version that lacks the Cox6b protein and possibly other subunits. However, in mitochondria from potato tubers and stems, about 90% of complex IV was present in monomeric form. It was suggested that the I(1)III(2)IV(4) supercomplex represents a basic unit for respiration in mammalian mitochondria termed respirasome. Respirasomes also occur in potato mitochondria but were of low concentrations under all conditions applied. We speculate that respirasomes are more abundant under in vivo conditions.
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