ArticlePlant physiology2004
Functional analysis of the final steps of the 1-deoxy-D-xylulose 5-phosphate (DXP) pathway to isoprenoids in plants using virus-induced gene silencing.
Article in Plant physiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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46 citing papers in PubMed, 121 citations in OpenAlex.
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- NtispH as a key regulator of glandular trichome development and terpenoid metabolism in tobacco (Nicotiana tabacum).Journal of plant research · 2026Article
- Medicinal Potential ofPlants (Basel, Switzerland) · 2025Review
- Modeling the effect of daytime duration on the biosynthesis of terpenoid precursors.Frontiers in plant science · 2024Article
- HDR, the last enzyme in the MEP pathway, differently regulates isoprenoid biosynthesis in two woody plants.Plant physiology · 2023Article
- Changing biosynthesis of terpenoid percursors in rice through synthetic biology.Frontiers in plant science · 2023Article
- Deciphering aroma formation during flowering in nectar tree (Forestry research · 2023Article
- Differential Regulation of an OsIspH1, the Functional 4-Hydroxy-3-Methylbut-2-Enyl Diphosphate Reductase, for Photosynthetic Pigment Biosynthesis in Rice Leaves and Seeds.Frontiers in plant science · 2022Article
- Plastid methylerythritol phosphate pathway participates in the hypersensitive response-related cell death inFrontiers in plant science · 2022Article
- Disruption of a Rice Chloroplast-Targeted Gene OsHMBPP Causes a Seedling-Lethal Albino Phenotype.Rice (New York, N.Y.) · 2020Article
- The subcellular localization of two isopentenyl diphosphate isomerases in rice suggests a role for the endoplasmic reticulum in isoprenoid biosynthesis.Plant cell reports · 2020Article
- SEED CAROTENOID DEFICIENT Functions in Isoprenoid Biosynthesis via the Plastid MEP Pathway.Plant physiology · 2019Article
- An investigation of the3 Biotech · 2018Review
- Isolation and characterization of 4-hydroxy-3-methylbut-2-enyl diphosphate reductase gene from Botryococcus braunii, race B.Journal of plant research · 2018Article
- Towards a sustainable bio-based economy: Redirecting primary metabolism to new products with plant synthetic biology.Plant science : an international journal of experimental plant biology · 2018Review
- Molecular cloning and functional identification of a cDNA encoding 4-hydroxy-3-methylbut-2-enyl diphosphate reductase fromActa pharmaceutica Sinica. B · 2017Article
- Overexpression and Suppression ofFrontiers in plant science · 2017Article
- Induction of Terpene Biosynthesis in Berries of Microvine Transformed withFrontiers in plant science · 2017Article
- Transcriptome Sequencing Analysis Reveals a Difference in Monoterpene Biosynthesis between ScentedFrontiers in plant science · 2017Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
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Abstract
Isoprenoid biosynthesis in plant plastids occurs via the 1-deoxy-d-xylulose 5-phosphate (DXP) pathway. We used tobacco rattle virus (TRV) to posttranscriptionally silence the expression of the last two enzymes of this pathway, the IspG-encoded (E)-4-hydroxy-3-methylbut-2-enyl diphosphate synthase (HDS) and the IspH-encoded isopentenyl/dimethylallyl diphosphate synthase (IDDS), as well as isopentenyl/dimethylallyl diphosphate isomerase (IDI), the enzyme that interconverts IPP and DMAPP. TRV-IspG and TRV-IspH infected Nicotiana benthamiana plants had albino leaves that contained less than 4% of the chlorophyll and carotenoid pigments of control leaves. We applied [(13)C]DXP and [(14)C]DXP to silenced leaves and found that 2-C-methyl-d-erythritol 2,4-cyclodiphosphate accumulated in plants blocked at HDS while DXP, (E)-4-hydroxy-3-methylbut-2-enyl phosphate and (E)-2-methylbut-2-ene-1,4-diol accumulated in IDDS-blocked plants. Albino leaves from IspG- and IspH-silenced plants displayed a disorganized palisade mesophyll, reduced cuticle, fewer plastids, and disrupted thylakoid membranes. These findings demonstrate the participation of HDS and IDDS in the DXP pathway in plants, and support the view that plastid isoprenoid biosynthesis is metabolically and physically segregated from the mevalonate pathway. IDI-silenced plants had mottled white-pale green leaves with disrupted tissue and plastid structure, and showed an 80% reduction in pigments compared to controls. IPP pyrophosphatase activity was higher in chloroplasts isolated from IDI-silenced plants than in control plant chloroplasts. We suggest that a low level of isoprenoid biosynthesis via the DXP pathway can occur without IDI but that this enzyme is required for full function of the DXP pathway.
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