ArticleThe Plant cell2004
Tissue-specific gene silencing mediated by a naturally occurring chalcone synthase gene cluster in Glycine max.
Article in The Plant cell, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 104 papers.
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Who cites it
104 citing papers in PubMed, 212 citations in OpenAlex.
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- Distinct thresholds condition sense posttranscriptional gene silencing initiation and amplification.The Plant cell · 2026Article
- Transcriptomic and Metabolic Insight Into Flavonoid Biosynthesis Underlying Black and Yellow Seed Coat Color Variation in Soybean (Plant direct · 2026Article
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- Flavonoids and anthocyanins in seagrasses: implications for climate change adaptation and resilience.Frontiers in plant science · 2024Review
- Seed color in lettuce is determined by the LsTT2, LsCHS, and Ls2OGD genes from the flavonoid biosynthesis pathway.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2023Article
- Recent advances in the improvement of soybean seed traits by genome editing.Plant biotechnology (Tokyo, Japan) · 2023Article
- Cloning, expression and activity analysises of chalcone synthase genes inChinese herbal medicines · 2023Article
- A novel QTL associated with tolerance to cold-induced seed cracking in the soybean cultivar Toyomizuki.Breeding science · 2023Article
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- Identification of candidate genes for soybean seed coat-related traits using QTL mapping and GWAS.Frontiers in plant science · 2023Article
- The Key to the Future Lies in the Past: Insights from Grain Legume Domestication and Improvement Should Inform Future Breeding Strategies.Plant & cell physiology · 2022Review
- Genome-Wide Identification, Characterization, and Expression Analysis of CHS Gene Family Members inGenes · 2022Article
- Identification of ST1 reveals a selection involving hitchhiking of seed morphology and oil content during soybean domestication.Plant biotechnology journal · 2022Article
44 more citing papers are in PubMed but not listed here.
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Chalcone synthase, a key regulatory enzyme in the flavonoid pathway, constitutes an eight-member gene family in Glycine max (soybean). Three of the chalcone synthase (CHS) gene family members are arranged as inverted repeats in a 10-kb region, corresponding to the I locus (inhibitor). Spontaneous mutations of a dominant allele (I or i(i)) to a recessive allele (i) have been shown to delete promoter sequences, paradoxically increasing total CHS transcript levels and resulting in black seed coats. However, it is not known which of the gene family members contribute toward pigmentation and how this locus affects CHS expression in other tissues. We investigated the unusual nature of the I locus using four pairs of isogenic lines differing with respect to alleles of the I locus. RNA gel blots using a generic open reading frame CHS probe detected similar CHS transcript levels in stems, roots, leaves, young pods, and cotyledons of the yellow and black isolines but not in the seed coats, which is consistent with the dominant I and i(i) alleles mediating CHS gene silencing in a tissue-specific manner. Using real-time RT-PCR, a variable pattern of expression of CHS genes in different tissues was demonstrated. However, increase in pigmentation in the black seed coats was associated with release of the silencing effect specifically on CHS7/CHS8, which occurred at all stages of seed coat development. These expression changes were linked to structural changes taking place at the I locus, shown to encompass a much wider region of at least 27 kb, comprising two identical 10.91-kb stretches of CHS gene duplications. The suppressive effect of this 27-kb I locus in a specific tissue of the G. max plant represents a unique endogenous gene silencing mechanism.
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