ArticleMolecular biology of the cell2004
High- and low-mobility populations of HP1 in heterochromatin of mammalian cells.
Article in Molecular biology of the cell, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 85 papers.
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Who cites it
85 citing papers in PubMed, 161 citations in OpenAlex.
- The basic domain of Suv39h2 buffers mitoxantrone-induced heterochromatin destabilization.iScience · 2026Article
- Unraveling the complexity of the histone code: implications for gene regulation and disease.Genome biology · 2026Review
- Understanding how genetically encoded tags and crowding agents affect phase separation by heterochromatin protein HP1α.Cell reports methods · 2025Article
- Heterochromatin protein 1 alpha (HP1α) undergoes a monomer to dimer transition that opens and compacts live cell genome architecture.Nucleic acids research · 2024Article
- Tracking live-cell single-molecule dynamics enables measurements of heterochromatin-associated protein-protein interactions.Nucleic acids research · 2024Article
- CFDP1 regulates the stability of pericentric heterochromatin thereby affecting RAN GTPase activity and mitotic spindle formation.PLoS biology · 2024Article
- From the membrane to the nucleus: mechanical signals and transcription regulation.Biophysical reviews · 2023Review
- Relationship between lysine methyltransferase levels and heterochromatin gene repression in living cells and in silico.PNAS nexus · 2023Article
- HP1 proteins regulate nucleolar structure and function by secluding pericentromeric constitutive heterochromatin.Nucleic acids research · 2023Article
- Confined migration induces heterochromatin formation and alters chromatin accessibility.iScience · 2022Article
- Article
- Live Cell Imaging of Nuclear Actin Filaments and Heterochromatic Repair foci in Drosophila and Mouse Cells.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Biology and Physics of Heterochromatin-Cells · 2020Review
- On the relations of phase separation and Hi-C maps to epigenetics.Royal Society open science · 2020Article
- Chromatin condensation fluctuations rather than steady-state predict chromatin accessibility.Nucleic acids research · 2019Article
- Citrullination of HP1γ chromodomain affects association with chromatin.Epigenetics & chromatin · 2019Article
- The interplay between H2A.Z and H3K9 methylation in regulating HP1α binding to linker histone-containing chromatin.Nucleic acids research · 2018Article
- Optimizing fluorescent protein expression for quantitative fluorescence microscopy and spectroscopy using herpes simplex thymidine kinase promoter sequences.FEBS open bio · 2018Article
- HP1α targets the chromosomal passenger complex for activation at heterochromatin before mitotic entry.The EMBO journal · 2018Article
- Epigenetic targeting drugs potentiate chemotherapeutic effects in solid tumor therapy.Scientific reports · 2017Article
25 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Heterochromatin protein 1 (HP1) is a conserved nonhistone chromosomal protein with functions in euchromatin and heterochromatin. Here we investigated the diffusional behaviors of HP1 isoforms in mammalian cells. Using fluorescence correlation spectroscopy (FCS) and fluorescence recovery after photobleaching (FRAP) we found that in interphase cells most HP1 molecules (50-80%) are highly mobile (recovery halftime: t(1/2) approximately 0.9 s; diffusion coefficient: D approximately 0.6-0.7 microm(2) s(-1)). Twenty to 40% of HP1 molecules appear to be incorporated into stable, slow-moving oligomeric complexes (t(1/2) approximately 10 s), and constitutive heterochromatin of all mammalian cell types analyzed contain 5-7% of very slow HP1 molecules. The amount of very slow HP1 molecules correlated with the chromatin condensation state, mounting to more than 44% in condensed chromatin of transcriptionally silent cells. During mitosis 8-14% of GFP-HP1alpha, but not the other isoforms, are very slow within pericentromeric heterochromatin, indicating an isoform-specific function of HP1alpha in heterochromatin of mitotic chromosomes. These data suggest that mobile as well as very slow populations of HP1 may function in concert to maintain a stable conformation of constitutive heterochromatin throughout the cell cycle.
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