ArticleMolecular biology of the cell2004
The T-stem determines the cytosolic or mitochondrial localization of trypanosomal tRNAsMet.
Article in Molecular biology of the cell, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 27 citations in OpenAlex.
- Non-coding RNA Regulated Cross-Talk Between Mitochondria and Other Cellular Compartments.Frontiers in cell and developmental biology · 2021Review
- Import of Non-Coding RNAs into Human Mitochondria: A Critical Review and Emerging Approaches.Cells · 2019Review
- tRNAs and proteins use the same import channel for translocation across the mitochondrial outer membrane of trypanosomes.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- tRNA biology in mitochondria.International journal of molecular sciences · 2015Review
- Mitochondrial membrane complex that contains proteins necessary for tRNA import in Trypanosoma brucei.The Journal of biological chemistry · 2012Article
- In vivo study in Trypanosoma brucei links mitochondrial transfer RNA import to mitochondrial protein import.EMBO reports · 2011Article
- tRNASec is transcribed by RNA polymerase II in Trypanosoma brucei but not in humans.Nucleic acids research · 2010Article
- Evolution of macromolecular import pathways in mitochondria, hydrogenosomes and mitosomes.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2010Review
- Thiolated tRNAs of Trypanosoma brucei are imported into mitochondria and dethiolated after import.The Journal of biological chemistry · 2009Article
- Elongation factor 1a mediates the specificity of mitochondrial tRNA import in T. brucei.The EMBO journal · 2007Article
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The mitochondrion of Trypanosoma brucei lacks tRNA genes. Organellar translation therefore depends on import of cytosolic, nucleus-encoded tRNAs. Except for the cytosol-specific initiator tRNA(Met), all trypanosomal tRNAs function in both the cytosol and the mitochondrion. The initiator tRNA(Met) is closely related to the imported elongator tRNA(Met). Thus, the distinct localization of the two tRNAs(Met) must be specified by the 26 nucleotides, which differ between the two molecules. Using transgenic T. brucei cell lines and subsequent cell fractionation, we show that the T-stem is both required and sufficient to specify the localization of the tRNAs(Met). Furthermore, it was shown that the tRNA(Met) T-stem localization determinants are also functional in the context of two other tRNAs. In vivo analysis of the modified nucleotides found in the initiator tRNA(Met) indicates that the T-stem localization determinants do not require modified nucleotides. In contrast, import of native tRNAs(Met) into isolated mitochondria suggests that nucleotide modifications might be involved in regulating the extent of import of elongator tRNA(Met).
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.