ArticleMolecular biology of the cell2004
Oda5p, a novel axonemal protein required for assembly of the outer dynein arm and an associated adenylate kinase.
Article in Molecular biology of the cell, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
39 citing papers in PubMed, 83 citations in OpenAlex.
- Changes in the Adenylate Kinase Activity are Proportional to the ADP/ATP Ratio Upon Resorption and Regeneration of Chlamydomonas reinhardtii Flagella.Cell biochemistry and biophysics · 2025Article
- Gene dosage of independent dynein arm motor preassembly factors influences cilia assembly in Chlamydomonas reinhardtii.PLoS genetics · 2024Article
- Review
- Adenylate kinase 9 is essential for sperm function and male fertility in mammals.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Cargo adapters expand the transport range of intraflagellar transport.Journal of cell science · 2022Review
- PKA, PP1, and DC1 phosphorylation mediate alcohol-induced ciliary dysfunction in Chlamydomonas reinhardtii.Alcohol (Fayetteville, N.Y.) · 2019Article
- Genetic causes of male infertility: snapshot on morphological abnormalities of the sperm flagellum.Basic and clinical andrology · 2019Article
- The flagellar protein Enkurin is required for mouse sperm motility and for transport through the female reproductive tract.Biology of reproduction · 2018Article
- Direction of flagellum beat propagation is controlled by proximal/distal outer dynein arm asymmetry.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Ciliary Motility: Regulation of Axonemal Dynein Motors.Cold Spring Harbor perspectives in biology · 2017Review
- Sperm dysfunction and ciliopathy.Reproductive medicine and biology · 2016Review
- Late steps in cytoplasmic maturation of assembly-competent axonemal outer arm dynein in Chlamydomonas require interaction of ODA5 and ODA10 in a complex.Molecular biology of the cell · 2015Article
- The CSC proteins FAP61 and FAP251 build the basal substructures of radial spoke 3 in cilia.Molecular biology of the cell · 2015Article
- The oligomeric outer dynein arm assembly factor CCDC103 is tightly integrated within the ciliary axoneme and exhibits periodic binding to microtubules.The Journal of biological chemistry · 2015Article
- Chlamydomonas axonemal dynein assembly locus ODA8 encodes a conserved flagellar protein needed for cytoplasmic maturation of outer dynein arm complexes.Cytoskeleton (Hoboken, N.J.) · 2015Article
- The ciliary inner dynein arm, I1 dynein, is assembled in the cytoplasm and transported by IFT before axonemal docking.Cytoskeleton (Hoboken, N.J.) · 2014Article
- CCDC151 mutations cause primary ciliary dyskinesia by disruption of the outer dynein arm docking complex formation.American journal of human genetics · 2014Article
- Adenine nucleotide metabolism and a role for AMP in modulating flagellar waveforms in mouse sperm.Biology of reproduction · 2014Article
- The awesome power of dikaryons for studying flagella and basal bodies in Chlamydomonas reinhardtii.Cytoskeleton (Hoboken, N.J.) · 2014Review
- Chlamydomonas ODA10 is a conserved axonemal protein that plays a unique role in outer dynein arm assembly.Molecular biology of the cell · 2013Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
Of the uncloned ODA genes required for outer dynein arm assembly in Chlamydomonas, ODA5 and ODA10 are of particular interest because they do not encode known subunits of the outer arm or the outer dynein arm-docking complex (ODA-DC), and because genetic studies suggest their products interact. Beginning with a tagged oda5 allele, we isolated genomic and cDNA clones of the wild-type gene. ODA5 predicts a novel, 66-kDa coiled-coil protein. Immunoblotting indicates Oda5p is an axonemal component that assembles onto the axoneme independently of the outer arm and ODA-DC and is uniquely missing in oda5 and oda10 axonemes. Oda5p is released from the axoneme by extraction with 0.6 M KCl, but the soluble Oda5p does not cosediment with the outer dynein arm/ODA-DC in sucrose gradients. Quantitative mass spectrometry by using isotope coded affinity tagging revealed that a previously unidentified adenylate kinase is reduced 35-50% in oda5 flagella. Direct enzymatic assays demonstrated a comparable reduction in adenylate kinase activity in oda5 flagella, and also in oda10 flagella, but not in flagella of other oda mutants. We propose that Oda5p is part of a novel axonemal complex that is required for outer arm assembly and anchors adenylate kinase in proximity to the arm.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.