ArticleMolecular and cellular biology2004
Thyroid hormones and gamma interferon specifically increase K15 keratin gene transcription.
Article in Molecular and cellular biology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed, 39 citations in OpenAlex.
- A Kaleidoscope of Keratin Gene Expression and the Mosaic of Its Regulatory Mechanisms.International journal of molecular sciences · 2023Review
- Associations of serum keratin 1 with thyroid function and immunity in Graves' disease.PloS one · 2023Article
- Topical L-thyroxine: The Cinderella among hormones waiting to dance on the floor of dermatological therapy?Experimental dermatology · 2020Review
- Expression of keratin 15 in dentigerous cyst, odontogenic keratocyst and ameloblastoma.Molecular and clinical oncology · 2019Article
- Thyroid diseases and skin autoimmunity.Reviews in endocrine & metabolic disorders · 2018Review
- E-cadherin roles in animal biology: A perspective on thyroid hormone-influence.Cell communication and signaling : CCS · 2016Review
- Perlecan expression influences the keratin 15-positive cell population fate in the epidermis of aging skin.Aging · 2016Article
- Proinflammatory Cytokines and Antiskin Autoantibodies in Patients With Inherited Epidermolysis Bullosa.Medicine · 2015Observational
- Transcriptional regulation analysis and the potential transcription regulator site in the extended KAP6.1 promoter in sheep.Molecular biology reports · 2014Article
- Keratin k15 as a biomarker of epidermal stem cells.International journal of molecular sciences · 2013Review
- The deiodinases and the control of intracellular thyroid hormone signaling during cellular differentiation.Biochimica et biophysica acta · 2013Review
- An Intimate Relationship between Thyroid Hormone and Skin: Regulation of Gene Expression.Frontiers in endocrinology · 2013Article
- Two mechanisms regulate keratin K15 expression in keratinocytes: role of PKC/AP-1 and FOXM1 mediated signalling.PloS one · 2012Article
- The thyroid hormone receptors as modulators of skin proliferation and inflammation.The Journal of biological chemistry · 2011Article
- Article
- Intermediate filaments take the heat as stress proteins.Trends in cell biology · 2010Review
- Epidermolysis bullosa simplex: a paradigm for disorders of tissue fragility.The Journal of clinical investigation · 2009Review
- Nexus between epidermolysis bullosa and transcriptional regulation by thyroid hormone in epidermal keratinocytes.Clinical and translational science · 2008Article
- Targeted skin overexpression of the mineralocorticoid receptor in mice causes epidermal atrophy, premature skin barrier formation, eye abnormalities, and alopecia.The American journal of pathology · 2007Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
Abstract
Basal layers of stratified epithelia express keratins K5, K14, and K15, which assemble into intermediate filament networks. Mutations in K5 or K14 genes cause epidermolysis bullosa simplex (EBS), a disorder with blistering in the basal layer due to cell fragility. Nonkeratinizing stratified epithelia, e.g., in the esophagus, produce more keratin K15 than epidermis, which alleviates the esophageal symptoms in patients with K14 mutations. Hypothesizing that increasing the cellular content of K15 could compensate for the mutant K14 and thus ease skin blistering in K14 EBS patients, we cloned the promoter of the K15 gene and examined its transcriptional regulation. Using cotransfection, gel mobility shifts, and DNase I footprinting, we have identified the regulators of K15 promoter activity and their binding sites. We focused on those that can be manipulated with extracellular agents, transcription factors C/EBP, AP-1, and NF-kappaB, nuclear receptors for thyroid hormone, retinoic acid, and glucocorticoids, and the cytokine gamma interferon (IFN-gamma). We found that C/EBP-beta and AP-1 induced, while retinoic acid, glucocorticoid receptors, and NF-kappaB suppressed, the K15 promoter, along with other keratin gene promoters. However, the thyroid hormone and IFN-gamma uniquely and potently activated the K15 promoter. Using these agents, we could boost the amounts of K15 in human epidermis. Our findings suggest that treatments based on thyroid hormone and IFN-gamma could become effective agents in therapy for patients with EBS.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.