ArticleJournal of bacteriology2004
Conserved target for group II intron insertion in relaxase genes of conjugative elements of gram-positive bacteria.
Article in Journal of bacteriology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 30 citations in OpenAlex.
- Group II Introns Generate Functional Chimeric Relaxase Enzymes with Modified Specificities through Exon Shuffling at Both the RNA and DNA Level.Molecular biology and evolution · 2021Article
- Enterococcal Genetics.Microbiology spectrum · 2019Review
- Article
- Mobile Group II Introns as Ancestral Eukaryotic Elements.Trends in genetics : TIG · 2017Review
- Recent horizontal transfer, functional adaptation and dissemination of a bacterial group II intron.BMC evolutionary biology · 2016Article
- Enterococcus faecalis PrgJ, a VirB4-like ATPase, mediates pCF10 conjugative transfer through substrate binding.Journal of bacteriology · 2012Article
- Cargo capacity of phages and plasmids and other factors influencing horizontal transfers of prokaryote transposable elements.Mobile genetic elements · 2012Article
- Article
- Distribution, diversity, and potential mobility of extrachromosomal elements related to the Bacillus anthracis pXO1 and pXO2 virulence plasmids.Applied and environmental microbiology · 2009Article
- The peptide pheromone-inducible conjugation system of Enterococcus faecalis plasmid pCF10: cell-cell signalling, gene transfer, complexity and evolution.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2007Review
- Specificity determinants of conjugative DNA processing in the Enterococcus faecalis plasmid pCF10 and the Lactococcus lactis plasmid pRS01.Molecular microbiology · 2007Article
- Genetic characterization of the conjugative DNA processing system of enterococcal plasmid pCF10.Plasmid · 2006Article
- Pheromone-inducible conjugation in Enterococcus faecalis: a model for the evolution of biological complexity?International journal of medical microbiology : IJMM · 2006Review
- Conjugative transfer of the Lactococcus lactis chromosomal sex factor promotes dissemination of the Ll.LtrB group II intron.Journal of bacteriology · 2005Article
- Characterization of the pheromone response of the Enterococcus faecalis conjugative plasmid pCF10: complete sequence and comparative analysis of the transcriptional and phenotypic responses of pCF10-containing cells to pheromone induction.Journal of bacteriology · 2005Article
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4 authors at 1 institution in 1 country.
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Abstract
The lactococcal group II intron Ll.ltrB interrupts the ltrB relaxase gene within a region that encodes a conserved functional domain. Nucleotides essential for the homing of Ll.ltrB into an intronless version of ltrB are found exclusively at positions required to encode amino acids broadly conserved in a family of relaxase proteins of gram-positive bacteria. Two of these relaxase genes, pcfG from the enterococcal plasmid pCF10 and the ORF4 gene in the streptococcal conjugative transposon Tn5252, were shown to support Ll.ltrB insertion into the conserved motif at precisely the site predicted by sequence homology with ltrB. Insertion occurred through a mechanism indistinguishable from retrohoming. Splicing and retention of conjugative function was demonstrated for pCF10 derivatives containing intron insertions. Ll.ltrB targeting of a conserved motif of a conjugative element suggests a mechanism for group II intron dispersal among bacteria. Additional support for this mechanism comes from sequence analysis of the insertion sites of the E.c.I4 family of bacterial group II introns.
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