ArticleJournal of bacteriology2004
A 12-base-pair deletion in the flagellar master control gene flhC causes nonmotility of the pathogenic German sorbitol-fermenting Escherichia coli O157:H- strains.
Article in Journal of bacteriology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 53 citations in OpenAlex.
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- Lipopolysaccharide Core Truncation in InvasiveInternational journal of molecular sciences · 2024Article
- Safety Properties ofFoods (Basel, Switzerland) · 2023Review
- SARS-CoV-2 and Emerging Foodborne Pathogens: Intriguing Commonalities and Obvious Differences.Pathogens (Basel, Switzerland) · 2022Review
- Biotyping reveals loss of motility in two distinct Yersinia ruckeri lineages exclusive to Norwegian aquaculture.Journal of fish diseases · 2022Article
- Pathogenomes of Atypical Non-shigatoxigenicFrontiers in microbiology · 2020Article
- Differential transcriptome analysis of enterohemorrhagic Escherichia coli strains reveals differences in response to plant-derived compounds.BMC microbiology · 2019Article
- Virulence Gene Profiles and Clonal Relationships of Escherichia coli O26:H11 Isolates from Feedlot Cattle as Determined by Whole-Genome Sequencing.Applied and environmental microbiology · 2016Article
- Is Shiga Toxin-Negative Escherichia coli O157:H7 Enteropathogenic or Enterohemorrhagic Escherichia coli? Comprehensive Molecular Analysis Using Whole-Genome Sequencing.Journal of clinical microbiology · 2015Article
- Characterization of the pathogenome and phylogenomic classification of enteropathogenic Escherichia coli of the O157:non-H7 serotypes.Pathogens and disease · 2015Article
- Genomic diversity and fitness of E. coli strains recovered from the intestinal and urinary tracts of women with recurrent urinary tract infection.Science translational medicine · 2013Article
- Prevalence, distribution and evolutionary significance of the IS629 insertion element in the stepwise emergence of Escherichia coli O157:H7.BMC microbiology · 2011Article
- Genotype and phenotypes of an intestine-adapted Escherichia coli K-12 mutant selected by animal passage for superior colonization.Infection and immunity · 2011Article
- Genome signatures of Escherichia coli O157:H7 isolates from the bovine host reservoir.Applied and environmental microbiology · 2011Article
- Divergent evolution and purifying selection of the flaA gene sequences in Aeromonas.Biology direct · 2009Article
- Liquid chromatography/mass spectrometry characterization of Escherichia coli and Shigella species.Journal of the American Society for Mass Spectrometry · 2008Article
- Genetic diversity among clonal lineages within Escherichia coli O157:H7 stepwise evolutionary model.Emerging infectious diseases · 2007Article
- Role of motility and the flhDC Operon in Escherichia coli MG1655 colonization of the mouse intestine.Infection and immunity · 2007Article
- Probing genomic diversity and evolution of Escherichia coli O157 by single nucleotide polymorphisms.Genome research · 2006Article
- The Escherichia coli O157 flagellar regulatory gene flhC and not the flagellin gene fliC impacts colonization of cattle.Infection and immunity · 2006Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
An atypical, Stx2-producing, pathogenic Escherichia coli O157:H(-) strain has been isolated with increasing frequency from hemolytic uremic syndrome patients in Germany. The lack of the H7 antigen coupled with the strain's ability to ferment sorbitol and express beta-glucuronidase have complicated its detection and identification. In this study, we have determined that the loss of motility in these German sorbitol-fermenting (SF) O157 strains is due to a 12-bp in-frame deletion in flhC that is required for transcriptional activation of genes involved in flagellum biosynthesis. Either complementation with a functional flhC or repair of this mutation restored H7 antigen expression and motility. PCR analysis of several nonmotile E. coli O157 strains from various geographical sources confirmed that the 12-bp flhC deletion is found only in the cluster of German SF O157 strains, providing a potentially useful marker by which these atypical strains can be identified. The loss of motility via mutations in the flhDC operon that we observed in the German SF O157 strains is consistent with a similar phenomenon currently observed in a significant subset of other important gram-negative pathogens.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.