ArticleJournal of bacteriology2004
Characterization of the cis-acting regulatory element controlling HrpB-mediated activation of the type III secretion system and effector genes in Ralstonia solanacearum.
Article in Journal of bacteriology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 55 papers.
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Who cites it
55 citing papers in PubMed, 111 citations in OpenAlex.
- Identification of protein secretion systems and type III effectors in wood-associated bacteria of the genusMicrobiology spectrum · 2026Article
- Prevalence and diversity of TAL effector-like proteins in fungal endosymbioticMicrobial genomics · 2024Article
- From prediction to function: Current practices and challenges towards the functional characterization of type III effectors.Frontiers in microbiology · 2023Review
- Complete genome sequence of an Israeli isolate ofFrontiers in plant science · 2023Article
- Comparative genomic analysis ofFrontiers in plant science · 2023Article
- Expression of theBiology · 2022Article
- The NEL Family of Bacterial E3 Ubiquitin Ligases.International journal of molecular sciences · 2022Review
- Plant immunity: Rice XA21-mediated resistance to bacterial infection.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Molecular identification and biological control ofSaudi journal of biological sciences · 2021Article
- Bacterial effectors mimicking ubiquitin-proteasome pathway tweak plant immunity.Microbiological research · 2021Review
- Implication of the Type III Effector RipS1 in the Cool-Virulence ofFrontiers in plant science · 2021Article
- The large, diverse, and robust arsenal of Ralstonia solanacearum type III effectors and their in planta functions.Molecular plant pathology · 2020Review
- Host-Pathogen Interactions betweenMicroorganisms · 2020Article
- A TAL effector-like protein of an endofungal bacterium increases the stress tolerance and alters the transcriptome of the host.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Insights into the Root Invasion by the Plant Pathogenic BacteriumPlants (Basel, Switzerland) · 2020Review
- Article
- Show me your secret(ed) weapons: a multifaceted approach reveals a wide arsenal of type III-secreted effectors in the cucurbit pathogenic bacterium Acidovorax citrulli and novel effectors in the Acidovorax genus.Molecular plant pathology · 2020Article
- Nosocomial bloodstream infection and the emerging carbapenem-resistant pathogen Ralstonia insidiosa.BMC infectious diseases · 2019Article
- Complete Genome Sequence of Sequevar 14MFrontiers in microbiology · 2019Article
- A modular toolbox for Golden-Gate-based plasmid assembly streamlines the generation of Ralstonia solanacearum species complex knockout strains and multi-cassette complementation constructs.Molecular plant pathology · 2018Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The ability of Ralstonia solanacearum to cause disease on plants depends on its type III secretion system (TTSS) encoded by hrp genes. The expression of hrp genes and known TTSS substrates is coordinately regulated by HrpB, a member of the AraC family of transcriptional regulators. Two HrpB-regulated promoters (hrpY and popABC) were characterized by deletion analysis, and the HrpB-dependent activation of these promoters was found to be conferred by a 25-nucleotide DNA element, the hrp(II) box (TTCGn16TTCG), which is present in other hrp promoters. The hrp(II) box element is an imperfect plant inducible promoter box, an element which was originally found in hrp promoters of Xanthomonas campestris (S. Fenselau and U. Bonas, Mol. Plant-Microbe Interact. 8:845-854, 1995) but which was not characterized at the molecular level. Site-directed mutagenesis showed that the hrp(II) box is essential for hrpY promoter activation in vivo. Functional analysis of the hrp(II) box element identified critical parameters that are required for HrpB-dependent activity. Further mapping analyses of several other hrpB-dependent promoters also indicated that the position of the hrp(II) box is conserved, at -70 to -47 bp from the transcriptional start. As a first step toward identifying novel TTSS effectors, we used the hrp(II) box consensus sequence to search for potential HrpB-regulated promoters in the complete genome sequence of R. solanacearum strain GMI1000. Among the 114 genes identified, a subset of promoters was found to have a structural relationship with hrp promoters, thus providing a pool of candidate genes encoding TTSS effectors.
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