ArticleJournal of bacteriology2004
Transgenic expression of RecA of the spirochetes Borrelia burgdorferi and Borrelia hermsii in Escherichia coli revealed differences in DNA repair and recombination phenotypes.
Article in Journal of bacteriology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
13 citing papers in PubMed, 17 citations in OpenAlex.
- Genetic Manipulation ofCurrent issues in molecular biology · 2021Review
- Article
- Functional analysis of Borrelia burgdorferi uvrA in DNA damage protection.FEMS microbiology letters · 2011Article
- Investigation of the genes involved in antigenic switching at the vlsE locus in Borrelia burgdorferi: an essential role for the RuvAB branch migrase.PLoS pathogens · 2009Article
- Borrelia burgdorferi vlsE antigenic variation is not mediated by RecA.Infection and immunity · 2008Article
- Differential telomere processing by Borrelia telomere resolvases in vitro but not in vivo.Journal of bacteriology · 2006Article
- A recA null mutation may be generated in Streptomyces coelicolor.Journal of bacteriology · 2006Article
- Antigenic variation by Borrelia hermsii occurs through recombination between extragenic repetitive elements on linear plasmids.Molecular microbiology · 2006Article
- Function and evolution of plasmid-borne genes for pyrimidine biosynthesis in Borrelia spp.Journal of bacteriology · 2006Article
- Horizontally acquired genes for purine salvage in Borrelia spp. causing relapsing fever.Infection and immunity · 2005Article
- Analysis of promoter elements involved in the transcriptional initiation of RpoS-dependent Borrelia burgdorferi genes.Journal of bacteriology · 2004Article
- Functional properties of Borrelia burgdorferi recA.Journal of bacteriology · 2004Article
- Genetic Manipulation of Borrelia Spp.Current topics in microbiology and immunologyReview
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
After unsuccessful attempts to recover a viable RecA-deficient mutant of the Lyme borreliosis agent Borrelia burgdorferi, we characterized the functional activities of RecA of B. burgdorferi, as well as RecA of the relapsing fever spirochete Borrelia hermsii and the free-living spirochete Leptospira biflexa, in a recA mutant of Escherichia coli. As a control, E. coli RecA was expressed from the same plasmid vector. DNA damage repair activity was assessed after exposure of the transgenic cells to UV light or the radiomimetic chemicals methyl methanesulfonate and mitomycin C. Recombination activity in the cells was assessed by using an assay for homologous recombination between repeats in the chromosome and by measuring the ability of the cells to foster lytic growth by red gam mutant bacteriophage lambda. Overall, we found that transgenic cells with recA genes of B. burgdorferi, B. hermsii, and L. biflexa had approximately equivalent activities in promoting homologous recombination in the lacZ duplication assay, but cells with B. burgdorferi recA and, most notably, B. hermsii recA were significantly less capable than cells with L. biflexa recA or E. coli recA in responding to DNA damage or in facilitating plaque formation in the phage assay. The comparatively poor function of Borrelia recA in the latter set of assays may be the consequence of impaired coordination in the loading of the transgenic RecA by RecBCD and/or RecFOR in E. coli.
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