ArticleGenes & development2004
A G alpha-dependent pathway that antagonizes multiple chemoattractant responses that regulate directional cell movement.
Article in Genes & development, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed.
- DPF is a cell-density sensing factor, with cell-autonomous and non-autonomous functions during Dictyostelium growth and development.BMC biology · 2019Article
- G-Protein Dependent Signal Transduction and Ubiquitination in Dictyostelium.International journal of molecular sciences · 2017Review
- A Worldwide Competition to Compare the Speed and Chemotactic Accuracy of Neutrophil-Like Cells.PloS one · 2016Article
- Phosphorylation of chemoattractant receptors regulates chemotaxis, actin reorganization and signal relay.Journal of cell science · 2013Article
- Glycogen Synthase Kinase 3 influences cell motility and chemotaxis by regulating PI3K membrane localization in Dictyostelium.Development, growth & differentiation · 2013Article
- The G alpha subunit Gα8 inhibits proliferation, promotes adhesion and regulates cell differentiation.Developmental biology · 2013Article
- Chemoattractant stimulation of TORC2 is regulated by receptor/G protein-targeted inhibitory mechanisms that function upstream and independently of an essential GEF/Ras activation pathway in Dictyostelium.Molecular biology of the cell · 2013Article
- A secreted protein is an endogenous chemorepellant in Dictyostelium discoideum.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Combinatorial cell-specific regulation of GSK3 directs cell differentiation and polarity in Dictyostelium.Development (Cambridge, England) · 2011Article
- Eukaryotic chemotaxis: a network of signaling pathways controls motility, directional sensing, and polarity.Annual review of biophysics · 2010Review
- A Dictyostelium chalone uses G proteins to regulate proliferation.BMC biology · 2009Article
- A protein with similarity to PTEN regulates aggregation territory size by decreasing cyclic AMP pulse size during Dictyostelium discoideum development.Eukaryotic cell · 2008Article
- Activation of Galphai3 triggers cell migration via regulation of GIV.The Journal of cell biology · 2008Article
- Oscillatory signaling and network responses during the development of Dictyostelium discoideum.Ageing research reviews · 2008Review
- G protein signaling governing cell fate decisions involves opposing Galpha subunits in Cryptococcus neoformans.Molecular biology of the cell · 2007Article
- Locally controlled inhibitory mechanisms are involved in eukaryotic GPCR-mediated chemosensing.The Journal of cell biology · 2007Article
- Nonadaptive regulation of ERK2 in Dictyostelium: implications for mechanisms of cAMP relay.Molecular biology of the cell · 2006Article
- Phosphoinositide 3-kinase activity controls the chemoattractant-mediated activation and adaptation of adenylyl cyclase.Molecular biology of the cell · 2006Article
- Adenylyl cyclase stimulation by cyclic adenosine monophosphate in the infusorian Dileptus anser cell culture.Doklady. Biochemistry and biophysicsArticle
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
Chemotactic cells, including neutrophils and Dictyostelium discoideum, orient and move directionally in very shallow chemical gradients. As cells polarize, distinct structural and signaling components become spatially constrained to the leading edge or rear of the cell. It has been suggested that complex feedback loops that function downstream of receptor signaling integrate activating and inhibiting pathways to establish cell polarity within such gradients. Much effort has focused on defining activating pathways, whereas inhibitory networks have remained largely unexplored. We have identified a novel signaling function in Dictyostelium involving a Galpha subunit (Galpha9) that antagonizes broad chemotactic response. Mechanistically, Galpha9 functions rapidly following receptor stimulation to negatively regulate PI3K/PTEN, adenylyl cyclase, and guanylyl cyclase pathways. The coordinated activation of these pathways is required to establish the asymmetric mobilization of actin and myosin that typifies polarity and ultimately directs chemotaxis. Most dramatically, cells lacking Galpha9 have extended PI(3,4,5)P(3), cAMP, and cGMP responses and are hyperpolarized. In contrast, cells expressing constitutively activated Galpha9 exhibit a reciprocal phenotype. Their second message pathways are attenuated, and they have lost the ability to suppress lateral pseudopod formation. Potentially, functionally similar Galpha-mediated inhibitory signaling may exist in other eukaryotic cells to regulate chemoattractant response.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.