ArticlePflugers Archiv : European journal of physiology2004
Effects of chloride and potassium channel blockers on apoptotic cell shrinkage and apoptosis in cortical neurons.
Article in Pflugers Archiv : European journal of physiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed, 94 citations in OpenAlex.
- Article
- Effects of Low- and High-Dose Valproic Acid and Lamotrigine on the Heart in Female Rats.Cardiovascular toxicology · 2022Article
- Low pH Attenuates Apoptosis by Suppressing the Volume-Sensitive Outwardly Rectifying (VSOR) Chloride Current in Chondrocytes.Frontiers in cell and developmental biology · 2021Article
- Dynamic changes in cell size and corresponding cell fate after optic nerve injury.Scientific reports · 2020Article
- Ions, the Movement of Water and the Apoptotic Volume Decrease.Frontiers in cell and developmental biology · 2020Review
- A Reverse-Osmosis Model of Apoptotic Shrinkage.Frontiers in cell and developmental biology · 2020Article
- Disruption of KNeuroscience · 2017Article
- Chloride channel protein 2 prevents glutamate-induced apoptosis in retinal ganglion cells.Iranian journal of basic medical sciences · 2016Article
- Cellular mechanisms of mutations in Kv7.1: auditory functions in Jervell and Lange-Nielsen syndrome vs. Romano-Ward syndrome.Frontiers in cellular neuroscience · 2015Article
- Functional significance of K+ channel β-subunit KCNE3 in auditory neurons.The Journal of biological chemistry · 2014Article
- Downregulation of chloride channel ClC-2 by Janus kinase 3.The Journal of membrane biology · 2014Article
- Voltage-gated potassium channels at the crossroads of neuronal function, ischemic tolerance, and neurodegeneration.Translational stroke research · 2014Review
- A voltage dependent non-inactivating Na+ channel activated during apoptosis in Xenopus oocytes.PloS one · 2014Article
- CrossTalk opposing view: The triggering and progression of the cell death machinery can occur without cell volume perturbations.The Journal of physiology · 2013Article
- ClC-3 chloride channel in hippocampal neuronal apoptosis.Neural regeneration research · 2013Article
- Convergent Ca2+ and Zn2+ signaling regulates apoptotic Kv2.1 K+ currents.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Translocation of PKC by yessotoxin in an in vitro model of Alzheimer's disease with improvement of tau and β-amyloid pathology.ACS chemical neuroscience · 2013Article
- Effect of chloride channel inhibitors on cytosolic Ca2+ levels and Ca2+-activated K+ (Gardos) channel activity in human red blood cells.The Journal of membrane biology · 2013Article
- Downregulation of KCNQ4 by Janus kinase 2.The Journal of membrane biology · 2013Article
- Phenomics of cardiac chloride channels.Comprehensive Physiology · 2013Review
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
K+ and Cl- homeostasis have been implicated in cell volume regulation and apoptosis. We addressed the hypothesis that K+ and Cl- efflux may contribute to apoptotic cell shrinkage and apoptotic death in cultured cortical neurons. CLC-2 and CLC-3 chloride channels were detected in cultured cortical neurons. The Cl- channel blockers 4,4'-diisothiocyanatostilbene-2,2'-disulfonic acid (DIDS), 4-acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic acid (SITS) and 5-nitro-2-(3-phenylpropylamino)-benzoic acid (NPPB) inhibited the outwardly rectifying Cl- current, prevented apoptotic cell shrinkage, and mildly attenuated cell death induced by staurosporine, C2-ceramide, or serum deprivation. Cl- channel blockers, however, at concentrations that prevented cell shrinkage had no significant effects on caspase activation and/or DNA fragmentation. Cell death in the presence of a Cl- channel blocker was still sensitive to blockade by the caspase inhibitor z-Val-Ala-Asp(OMe)-fluoromethyl ketone (z-VAD-fmk). Electron microscopy revealed that, although DIDS prevented apoptotic cell shrinkage, certain apoptotic ultrastructural alterations still took place in injured neurons. On the other hand, the K+ channel blocker tetraethylammonium (TEA), clofilium, or the caspase inhibitor z-VAD-fmk prevented cell shrinkage as well as caspase activation and/or DNA damage, and showed stronger neuroprotection against apoptotic alterations and cell death. The results indicate that neurons may undergo apoptotic process without cell shrinkage and imply distinct roles for Cl- and K+ homeostasis in regulating different apoptotic events.
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