ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2004
Arthropod 5-HT2 receptors: a neurohormonal receptor in decapod crustaceans that displays agonist independent activity resulting from an evolutionary alteration to the DRY motif.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed.
- Article
- Serotonergic modulation across sensory modalities.Journal of neurophysiology · 2020Review
- Serotonin in Animal Cognition and Behavior.International journal of molecular sciences · 2020Review
- Modulator-Gated, SUMOylation-Mediated, Activity-Dependent Regulation of Ionic Current Densities Contributes to Short-Term Activity Homeostasis.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2019Article
- Article
- Activation mechanism of a neuromodulator-gated pacemaker ionic current.Journal of neurophysiology · 2017Article
- From Blood to Brain: Adult-Born Neurons in the Crayfish Brain Are the Progeny of Cells Generated by the Immune System.Frontiers in neuroscience · 2017Review
- Dm5-HTFrontiers in systems neuroscience · 2017Article
- Deep sequencing of transcriptomes from the nervous systems of two decapod crustaceans to characterize genes important for neural circuit function and modulation.BMC genomics · 2016Article
- Fast-Scan Cyclic Voltammetry (FSCV) Detection of Endogenous Octopamine in Drosophila melanogaster Ventral Nerve Cord.ACS chemical neuroscience · 2016Article
- Identification of genes related to learning and memory in the brain transcriptome of the mollusc, Hermissenda crassicornis.Learning & memory (Cold Spring Harbor, N.Y.) · 2015Article
- Neuropeptide receptor transcript expression levels and magnitude of ionic current responses show cell type-specific differences in a small motor circuit.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2015Article
- Monoaminergic tone supports conductance correlations and stabilizes activity features in pattern generating neurons of the lobster, Panulirus interruptus.Frontiers in neural circuits · 2015Article
- Article
- Serotonin and synaptic transmission at invertebrate neuromuscular junctions.Experimental neurobiology · 2012Article
- Adult neurogenesis in the decapod crustacean brain: a hematopoietic connection?The European journal of neuroscience · 2011Review
- 5-HT receptors mediate lineage-dependent effects of serotonin on adult neurogenesis in Procambarus clarkii.Neural development · 2011Article
- Cell specific dopamine modulation of the transient potassium current in the pyloric network by the canonical D1 receptor signal transduction cascade.Journal of neurophysiology · 2010Article
- D(2) receptors receive paracrine neurotransmission and are consistently targeted to a subset of synaptic structures in an identified neuron of the crustacean stomatogastric nervous system.The Journal of comparative neurology · 2010Article
- Modulation of stomatogastric rhythms.Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology · 2009Review
Corrections and comments
- Erratum issued
Authors and funding
7 authors.
Funding
Abstract
The stomatogastric nervous system (STNS) is a premiere model for studying modulation of motor pattern generation. Whereas the cellular and network responses to monoamines have been particularly well characterized electrophysiologically, the transduction mechanisms that link the different monoaminergic signals to specific intracellular responses are presently unknown in this system. To begin to elucidate monoaminergic signal transduction in pyloric neurons, we used a bioinformatics approach to predict the existence of 18 monoamine receptors in arthropods, 9 of which have been previously cloned in Drosophila and other insects. We then went on to use the two existing insect databases to clone and characterize the 10th putative arthropod receptor from the spiny lobster, Panulirus interruptus. This receptor is most homologous to the 5-HT2 subtype and shows a dose-dependent response to 5-HT but not to any of the other monoamines present in the STNS. Through a series of pharmacological experiments, we demonstrate that this newly described receptor, 5-HT2betaPan, couples with the traditional G(q) pathway when expressed in HEK293 cells, but not to G(s) or G(i/o). Moreover, it is constitutively active, because the highly conserved DRY motif in transmembrane region 3 has evolved into DRF. Site-directed mutagenesis that reverts the motif back to DRY abolishes this agonist-independent activity. We further demonstrate that this receptor most likely participates in the modulation of stomatogastric motor output, because it is found in neurites in the synaptic neuropil of the stomatogastric ganglion as well as in the axon terminals at identified pyloric neuromuscular junctions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.