ArticleThe EMBO journal1992
A novel gene, spp91-1, suppresses the splicing defect and the pre-mRNA nuclear export in the prp9-1 mutant.
Article in The EMBO journal, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed.
- Proteins associated with SF3a60 in T. brucei.PloS one · 2014Article
- Functional Cus1p is found with Hsh155p in a multiprotein splicing factor associated with U2 snRNA.Molecular and cellular biology · 2000Article
- Article
- Induction and maintenance of autonomous flock house virus RNA1 replication.Journal of virology · 1999Article
- Purification of the yeast U4/U6.U5 small nuclear ribonucleoprotein particle and identification of its proteins.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
- Snt309p modulates interactions of Prp19p with its associated components to stabilize the Prp19p-associated complex essential for pre-mRNA splicing.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
- Cap-independent polysomal association of natural mRNAs encoding c-myc, BiP, and eIF4G conferred by internal ribosome entry sites.RNA (New York, N.Y.) · 1998Article
- Article
- Replication of flock house virus RNAs from primary transcripts made in cells by RNA polymerase II.Journal of virology · 1997Article
- Expression of a reporter gene interrupted by the Candida albicans group I intron is inhibited by base analogs.Nucleic acids research · 1997Article
- Article
- The PRP31 gene encodes a novel protein required for pre-mRNA splicing in Saccharomyces cerevisiae.Nucleic acids research · 1996Article
- Invariant U2 RNA sequences bordering the branchpoint recognition region are essential for interaction with yeast SF3a and SF3b subunits.Molecular and cellular biology · 1996Article
- An extragenic suppressor of prp24-1 defines genetic interaction between PRP24 and PRP21 gene products of Saccharomyces cerevisiae.Molecular & general genetics : MGG · 1996Article
- Autoregulation of expression of the yeast Dbp2p 'DEAD-box' protein is mediated by sequences in the conserved DBP2 intron.The EMBO journal · 1995Article
- Splicing factor SF3a60 is the mammalian homologue of PRP9 of S.cerevisiae: the conserved zinc finger-like motif is functionally exchangeable in vivo.Nucleic acids research · 1994Article
- SWAP pre-mRNA splicing regulators are a novel, ancient protein family sharing a highly conserved sequence motif with the prp21 family of constitutive splicing proteins.Nucleic acids research · 1994Article
- Interactions between highly conserved U2 small nuclear RNA structures and Prp5p, Prp9p, Prp11p, and Prp21p proteins are required to ensure integrity of the U2 small nuclear ribonucleoprotein in Saccharomyces cerevisiae.Molecular and cellular biology · 1994Article
- Interaction between the first and last nucleotides of pre-mRNA introns is a determinant of 3' splice site selection in S. cerevisiae.Nucleic acids research · 1994Article
- Analysis of autoregulation at the level of pre-mRNA splicing of the suppressor-of-white-apricot gene in Drosophila.Genetics · 1994Article
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2 authors.
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Abstract
Processing and export of nuclear pre-mRNA are believed to be competing processes in the nucleus. In order to identify factors which are involved in these processes, we isolated suppressors that relieve the growth defect of a prp9-1 temperature-sensitive mutant strain of Saccharomyces cerevisiae. The prp9-1 mutation was previously shown to abolish splicing and to target pre-mRNA to the cytoplasm. One of the suppressors, spp91-1, corrects the prp9-1 growth defect through partial restoration of splicing and by a complete reversion of the pre-mRNA escape phenotype. This suppressor is specific for two prp9 alleles and cannot substitute for PRP9 function. The mutant and wild-type alleles of SPP91 were cloned and sequenced. SPP91 encodes a novel protein essential for mitotic growth whose sequence contains motifs indicative of a nuclear localization. In vivo depletion of SPP91 in a prp9-1 genetic background is lethal and is associated with reduced amounts of spliced mRNA and accumulation of pre-mRNA. This observation strongly supports the hypothesis that SPP91 encodes a PRP factor. We suggest that spp91-1 increases pre-mRNA retention in the nucleus by improving the formation of the spliceosome and thereby allowing a larger proportion of the pre-mRNA molecules to be spliced.
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