Evidence map›Paper›PMID 15053484›Full record

ArticleChromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology2004

Chromosome paints from single copies of chromosomes.

Susan Gribble, Bee L Ng, Elena Prigmore, Deborah C Burford, Nigel P Carter

Abstract read
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In one paragraph

Article in Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.

0numbers the graph read from it
0cells of the map it votes in
15citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

15 citing papers in PubMed.

  1. Article
  2. B chromosome dynamics inComparative cytogenetics · 2017
    Article
  3. Origin of B chromosomes inComparative cytogenetics · 2017
    Article
  4. Article
  5. Article
  6. Article
  7. Article
  8. Article
  9. Chromosomes in the flow to simplify genome analysis.Functional & integrative genomics · 2012
    Review
  10. Whole genome amplification in preimplantation genetic diagnosis.Journal of Zhejiang University. Science. B · 2011
    Review
  11. Preparation of Xenopus tropicalis whole chromosome painting probes using laser microdissection and reconstruction of X. laevis tetraploid karyotype by Zoo-FISH.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2010
    Article
  12. Article
  13. Microdissection and chromosome painting of X and B chromosomes in Locusta migratoria.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2009
    Article
  14. Article
  15. Generation of chromosome painting probes from single chromosomes by laser microdissection and linker-adaptor PCR.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2004
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Susan GribbleThe Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, CB10 1SA, UK.
Bee L Ng
Elena Prigmore
Deborah C Burford
Nigel P Carter

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

We have used OmniPlex library technology to construct chromosome painting probes from single copies of flow sorted chromosomes. We show that this whole genome amplification technology is particularly efficient at amplifying single copies of chromosomes for the production of paints and that single aberrant chromosomes can be analysed in this way using reverse chromosome painting. The efficient generation of painting probes from single copies of sorted chromosomes has the advantage that the probe must be specific for the chromosome sorted and will not suffer from contamination from other chromosomes particularly in situations where flow karyotype peaks are poorly resolved. These initial results suggest that OmniPlex whole genome amplification will be equally effective in other cytogenetic applications where only small amounts of DNA are available, i.e. from single cells or from small pieces of microdissected tissue.

Indexed as

DNA ProbesChildChromosome AberrationsChromosome PaintingCytogeneticsFemaleFlow CytometryGenomeHumansDNA Probes

Identifiers

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.