ArticleThe Journal of experimental medicine2004
Embryonic stem cells as an alternate marrow donor source: engraftment without graft-versus-host disease.
Article in The Journal of experimental medicine, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
27 citing papers in PubMed, 111 citations in OpenAlex.
- Stem Cell Transplantation Therapy and Neurological Disorders: Current Status and Future Perspectives.Biology · 2022Review
- Healing the Broken Heart; The Immunomodulatory Effects of Stem Cell Therapy.Frontiers in immunology · 2020Review
- Concise Review: Recent Advances in the In Vitro Derivation of Blood Cell Populations.Stem cells translational medicine · 2016Review
- F4/80Journal of immunology research · 2016Article
- Improved Survival and Hematopoietic Differentiation of Murine Embryonic Stem Cells on Electrospun Polycaprolactone Nanofiber.Cell journal · 2016Article
- In vivo repopulating activity emerges at the onset of hematopoietic specification during embryonic stem cell differentiation.Stem cell reports · 2015Article
- De novo formed satellite DNA-based mammalian artificial chromosomes and their possible applications.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2015Review
- Mesodermal and hematopoietic differentiation from ES and iPS cells.Stem cell reviews and reports · 2013Review
- Generation of engraftable hematopoietic stem cells from induced pluripotent stem cells by way of teratoma formation.Molecular therapy : the journal of the American Society of Gene Therapy · 2013Article
- Hematopoietic cell differentiation from embryonic and induced pluripotent stem cells.Stem cell research & therapy · 2013Review
- Immunological applications of stem cells in type 1 diabetes.Endocrine reviews · 2011Review
- Embryonic stem cells in scaffold-free three-dimensional cell culture: osteogenic differentiation and bone generation.Head & face medicine · 2011Article
- The challenge of immunogenicity in the quest for induced pluripotency.Nature reviews. Immunology · 2010Review
- Induction of hematopoietic differentiation of mouse embryonic stem cells by an AGM-derived stromal cell line is not further enhanced by overexpression of HOXB4.Stem cells and development · 2010Article
- Hematopoiesis from pluripotent stem cell lines.International journal of hematology · 2010Review
- Autologous blood cell therapies from pluripotent stem cells.Blood reviews · 2010Review
- Twisting immune responses for allogeneic stem cell therapy.World journal of stem cells · 2009Article
- Myc-regulated microRNAs attenuate embryonic stem cell differentiation.The EMBO journal · 2009Article
- Surface antigen phenotypes of hematopoietic stem cells from embryos and murine embryonic stem cells.Blood · 2009Article
- HOXB4 but not BMP4 confers self-renewal properties to ES-derived hematopoietic progenitor cells.Transplantation · 2008Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A single embryonic stem cell (ESC) line can be repetitively cryopreserved, thawed, expanded, and differentiated into various cellular components serving as a potentially renewable and well-characterized stem cell source. Therefore, we determined whether ESCs could be used to reconstitute marrow and blood in major histocompatibility complex (MHC)-mismatched mice. To induce differentiation toward hematopoietic stem cells (HSCs) in vitro, ESCs were cultured in methylcellulose with stem cell factor, interleukin (IL)-3, and IL-6. ESC-derived, cytokine-induced HSCs (c-kit+/CD45+) were isolated by flow cytometry and injected either intra bone marrow or intravenously into lethally irradiated MHC-mismatched recipient mice. From 2 wk to 6 mo after injection, the peripheral blood demonstrated increasing ESC-derived mononuclear cells that included donor-derived T and B lymphocytes, monocytes, and granulocytes without clinical or histologic evidence of graft-versus-host disease (GVHD). Mixed lymphocyte culture assays demonstrated T cell tolerance to both recipient and donor but intact third party proliferative responses and interferon gamma production. ESCs might be used as a renewable alternate marrow donor source that reconstitutes hematopoiesis with intact immune responsiveness without GVHD despite crossing MHC barriers.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.