ArticleMolecular and cellular probes2004
Development of a p28-based PCR assay for Ehrlichia chaffeensis.
Article in Molecular and cellular probes, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 21 citations in OpenAlex.
- Molecular Investigation of the p28 Gene ofVeterinary medicine international · 2025Article
- New York State Tick Blitz: harnessing community-based science to understand range expansion of ticks.Journal of medical entomology · 2023Article
- Under-diagnosis of vector-borne diseases among individuals suspected of having Scrub Typhus in South Korea.PloS one · 2023Article
- Protozoan and Microbial Pathogens of House Cats in the Province of Tekirdag in Western Turkey.Pathogens (Basel, Switzerland) · 2021Article
- Experimental infection of Rhipicephalus sanguineus with Ehrlichia chaffeensis.Veterinary microbiology · 2014Article
- The prevalence of zoonotic tick-borne pathogens in Ixodes scapularis collected in the Hudson Valley, New York State.Vector borne and zoonotic diseases (Larchmont, N.Y.) · 2014Article
- Host surveys, ixodid tick biology and transmission scenarios as related to the tick-borne pathogen, Ehrlichia canis.Veterinary parasitology · 2008Review
- Experimental infection of dairy calves with Ehrlichia chaffeensis.Journal of medical microbiology · 2007Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
Detection of Ehrlichia chaffeensis is necessary to study interactions between the parasite and its vertebrate and invertebrate hosts. The purpose of this study was to develop a sensitive, specific PCR assay for E. chaffeensis based on the outer membrane protein gene, p28. Candidate primer sets were identified and ranked based on annealing scores, similarities to three major p28 sequence clusters, dissimilarity to E. canis p30, an ortholog of p28, and the proximities of flanking primer sequences for nested PCR. The relative sensitivities of five optimized single-step and two nested PCR assays were determined, and the most sensitive assay was found to be a single-step PCR that was as much as 1000-fold more sensitive than a standard 16S rDNA-based nested PCR assay. This p28-based PCR assay amplified the target amplicon from isolates representative of all three major clusters of known p28 sequences, and this assay did not amplify template prepared from either of the two species most closely related to E. chaffeensis, E. canis and E. muris. These results indicate that this sensitive, specific and isolate-universal single-step PCR assay will be a useful tool in characterizing the transmission of this important zoonotic pathogen.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.