ArticlePlant physiology2004
The effect of alpha-amanitin on the Arabidopsis seed proteome highlights the distinct roles of stored and neosynthesized mRNAs during germination.
Article in Plant physiology, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 160 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
160 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Seed Longevity in Legumes: Deeper Insights Into Mechanisms and Molecular Perspectives.Frontiers in plant science · 2022Pooled it
- Transcriptional activity during seed imbibition is conserved, robust and required for germination.Nature communications · 2026Article
- Replication-independent eviction of the histone variant H2B.8 reveals chromatin reprogramming during seed imbibition.Nature communications · 2026Article
- Holistic overview of regulatory networks governing seed dormancy and germination in plants.Science China. Life sciences · 2026Review
- Molecular timekeepers: the curious alliance of redox, repair, and protective proteins in preserving seed longevity.Crop health · 2026Review
- Adaptive transgenerational plasticity through priming in response to neighbor density in Mimulus platycalyx.PloS one · 2026Article
- Translational landscape during seed germination revealed by ribosome profiling.The Plant journal : for cell and molecular biology · 2026Article
- The phylotranscriptomic profile of angiosperm seed development follows a reverse hourglass pattern.The Plant cell · 2025Article
- Defying Death: A Multi-Omics Approach to Understanding Desiccation Tolerance and Senescence inPlants (Basel, Switzerland) · 2025Article
- GWAS and gene co-expression network analysis reveal the genetic control of seed germination under salt stress in maize.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2025Article
- Exogenous neoagaro-oligosaccharides accelerate seed germination and alleviate the impact of growth retardants on germination and seedling growth of garden cosmos (Cosmos bipinnatus Cav.).Scientific reports · 2025Article
- Natural variation in the ZmPIMT1 promoter enhances seed aging tolerance by regulating PABP2 repair in maize.The Plant cell · 2025Article
- Unravelling the dynamics of seed-stored mRNAs during seed priming.The New phytologist · 2025Article
- Control of seed-to-seedling transition by an upstream open reading frame inProceedings of the National Academy of Sciences of the United States of America · 2025Article
- Deciphering Seed Deterioration: Molecular Insights and Priming Strategies for Revitalizing Aged Seeds.Plants (Basel, Switzerland) · 2025Review
- The Regulatory Role of Exogenous Carnitine Applications in Lipid Metabolism, Mitochondrial Respiration, and Germination in Maize Seeds (Life (Basel, Switzerland) · 2025Article
- Establishment of single-cell transcriptional states during seed germination.Nature plants · 2024Article
- A commitment for life: Decades of unraveling the molecular mechanisms behind seed dormancy and germination.The Plant cell · 2024Review
- Interplay between coding and non-coding regulation drives the Arabidopsis seed-to-seedling transition.Nature communications · 2024Article
- Cycloheximide in the nanomolar range inhibits seed germination ofJournal of pesticide science · 2024Article
100 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To investigate the role of stored and neosynthesized mRNAs in seed germination, we examined the effect of alpha-amanitin, a transcriptional inhibitor targeting RNA polymerase II, on the germination of nondormant Arabidopsis seeds. We used transparent testa mutants, of which seed coat is highly permeable, to better ascertain that the drug can reach the embryo during seed imbibition. Even with the most permeable mutant (tt2-1), germination (radicle protrusion) occurred in the absence of transcription, while subsequent seedling growth was blocked. In contrast, germination was abolished in the presence of the translational inhibitor cycloheximide. Taken together, the results highlight the role of stored proteins and mRNAs for germination in Arabidopsis and show that in this species the potential for germination is largely programmed during the seed maturation process. The alpha-amanitin-resistant germination exhibited characteristic features. First, this germination was strongly slowed down, indicating that de novo transcription normally allows the synthesis of factor(s) activating the germination rate. Second, the sensitivity of germination to gibberellic acid was reduced 15-fold, confirming the role of this phytohormone in germination. Third, de novo synthesis of enzymes involved in reserve mobilization and resumption of metabolic activity was repressed, thus accounting for the failure in seedling establishment. Fourth, germinating seeds can recapitulate at least part of the seed maturation program, being capable of using mRNAs stored during development. Thus, commitment to germination and plant growth requires transcription of genes allowing the imbibed seed to discriminate between mRNAs to be utilized in germination and those to be destroyed.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.