ArticleNucleic acids research2004
Characterization of a novel RNA polymerase II arrest site which lacks a weak 3' RNA-DNA hybrid.
Article in Nucleic acids research, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 26 citations in OpenAlex.
- U1 snRNP increases RNA Pol II elongation rate to enable synthesis of long genes.Molecular cell · 2023Article
- PHF3 regulates neuronal gene expression through the Pol II CTD reader domain SPOC.Nature communications · 2021Article
- Sequence-Modified Antibiotic Resistance Genes Provide Sustained Plasmid-Mediated Transgene Expression in Mammals.Molecular therapy : the journal of the American Society of Gene Therapy · 2017Article
- Control of transcriptional pausing by biased thermal fluctuations on repetitive genomic sequences.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Bridge helix bending promotes RNA polymerase II backtracking through a critical and conserved threonine residue.Nature communications · 2016Article
- A Two-Way Street: Regulatory Interplay between RNA Polymerase and Nascent RNA Structure.Trends in biochemical sciences · 2016Review
- Real-Time Observation of Backtracking by Bacterial RNA Polymerase.Biochemistry · 2016Article
- Visualizing translocation dynamics and nascent transcript errors in paused RNA polymerases in vivo.Genome biology · 2015Article
- Unveiling translocation intermediates of RNA polymerase.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Transcription elongation. Heterogeneous tracking of RNA polymerase and its biological implications.Transcription · 2014Review
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- Factor-independent transcription pausing caused by recognition of the RNA-DNA hybrid sequence.The EMBO journal · 2012Article
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- Mechanisms and implications of transcription blockage by guanine-rich DNA sequences.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Mechanism of sequence-specific pausing of bacterial RNA polymerase.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Transcript elongation by RNA polymerase II is blocked at DNA sequences called arrest sites. An exceptionally weak RNA-DNA hybrid is often thought to be necessary at the point of arrest. We have identified an arrest site from the tyrosine hydroxylase (TH) gene which does not fit this pattern. Transcription of many sequence variants of this site shows that the RNA-DNA hybrid over the three bases immediately preceding the major arrest point may be strong (i.e. C:G) without interfering with arrest. However, arrest at the TH site requires the presence of a pyrimidine at the 3' end and arrest increases when the 3'-most segment is pyrimidine rich. We also demonstrated that arrest at the TH site is completely dependent on the presence of a purine-rich element immediately upstream of the RNA-DNA hybrid. Thus, the RNA polymerase II arrest element from the TH gene has several unanticipated characteristics: arrest is independent of a weak RNA-DNA hybrid at the 3' end of the transcript, but it requires both a pyrimidine at the 3' end and a polypurine element upstream of the RNA-DNA hybrid.
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