ReviewLeukemia2004
Split-signal FISH for detection of chromosome aberrations in acute lymphoblastic leukemia.
Review in Leukemia, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 73 citations in OpenAlex.
- Diagnostic Implications of NGS-Based Molecular Profiling in Mature B-Cell Lymphomas with Potential Bone Marrow Involvement.Diagnostics (Basel, Switzerland) · 2025Article
- A Fluorescent In Situ Hybridization (FISH) Assay for Detection of HBV RNA, DNA, and cccDNA in Cell Culture.Methods in molecular biology (Clifton, N.J.) · 2024Article
- The KMT2A recombinome of acute leukemias in 2023.Leukemia · 2023Article
- Cellular and genomic approaches for exploring structural chromosomal rearrangements.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2020Review
- Protein methyltransferase inhibitors as precision cancer therapeutics: a decade of discovery.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2018Review
- The MLL recombinome of acute leukemias in 2017.Leukemia · 2018Article
- The MLL recombinome of acute leukemias in 2013.Leukemia · 2013Article
- Double-staining chromogenic in situ hybridization as a useful alternative to split-signal fluorescence in situ hybridization in lymphoma diagnostics.Haematologica · 2010Article
- New insights to the MLL recombinome of acute leukemias.Leukemia · 2009Article
- Flow cytometric immunobead assay for the detection of BCR-ABL fusion proteins in leukemia patients.Leukemia · 2009Article
- E2A-ZNF384 and NOL1-E2A fusion created by a cryptic t(12;19)(p13.3; p13.3) in acute leukemia.Leukemia · 2008Article
- Prognostic significance of molecular-cytogenetic abnormalities in pediatric T-ALL is not explained by immunophenotypic differences.Leukemia · 2008Article
- Complex MLL rearrangements in t(4;11) leukemia patients with absent AF4.MLL fusion allele.Leukemia · 2007Article
- Acute lymphoblastic leukemia with t(4;11) in children 1 year and older: The 'big sister' of the infant disease?Leukemia · 2007Article
- Mapping of MYC breakpoints in 8q24 rearrangements involving non-immunoglobulin partners in B-cell lymphomas.Leukemia · 2007Article
- Correlated break at PARK2/FRA6E and loss of AF-6/Afadin protein expression are associated with poor outcome in breast cancer.Oncogene · 2007Article
- Article
- Molecular cytogenetics in haematological malignancy: current technology and future prospects.Chromosoma · 2005Review
- Diagnostic tool for the identification of MLL rearrangements including unknown partner genes.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Chromosome aberrations are frequently observed in precursor-B-acute lymphoblastic leukemias (ALL) and T-cell acute lymphoblastic leukemias (T-ALL). These translocations can form leukemia-specific chimeric fusion proteins or they can deregulate expression of an (onco)gene, resulting in aberrant expression or overexpression. Detection of chromosome aberrations is an important tool for risk classification. We developed rapid and sensitive split-signal fluorescent in situ hybridization (FISH) assays for six of the most frequent chromosome aberrations in precursor-B-ALL and T-ALL. The split-signal FISH approach uses two differentially labeled probes, located in one gene at opposite sites of the breakpoint region. Probe sets were developed for the genes TCF3 (E2A) at 19p13, MLL at 11q23, ETV6 at 12p13, BCR at 22q11, SIL-TAL1 at 1q32 and TLX3 (HOX11L2) at 5q35. In normal karyotypes, two colocalized green/red signals are visible, but a translocation results in a split of one of the colocalized signals. Split-signal FISH has three main advantages over the classical fusion-signal FISH approach, which uses two labeled probes located in two genes. First, the detection of a chromosome aberration is independent of the involved partner gene. Second, split-signal FISH allows the identification of the partner gene or chromosome region if metaphase spreads are present, and finally it reduces false-positivity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.