ArticleBiophysical journal2004
Longitudinal diffusion in retinal rod and cone outer segment cytoplasm: the consequence of cell structure.
Article in Biophysical journal, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
24 citing papers in PubMed.
- Photoreceptor disc incisures form as an adaptive mechanism ensuring the completion of disc enclosure.eLife · 2023Article
- Photoreceptor disc incisures form as an adaptive mechanism ensuring the completion of disc enclosure.bioRxiv : the preprint server for biology · 2023Article
- Analysis of waveform and amplitude of mouse rod and cone flash responses.The Journal of physiology · 2021Article
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- Article
- Multi-scale, numerical modeling of spatio-temporal signaling in cone phototransduction.PloS one · 2019Article
- Local, nonlinear effects of cGMP and Ca2+ reduce single photon response variability in retinal rods.PloS one · 2019Article
- In vivo optophysiology reveals that G-protein activation triggers osmotic swelling and increased light scattering of rod photoreceptors.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Molecular basis for photoreceptor outer segment architecture.Progress in retinal and eye research · 2016Review
- How rods respond to single photons: Key adaptations of a G-protein cascade that enable vision at the physical limit of perception.BioEssays : news and reviews in molecular, cellular and developmental biology · 2015Article
- cGMP in mouse rods: the spatiotemporal dynamics underlying single photon responses.Frontiers in molecular neuroscience · 2015Review
- Detection of single photons by toad and mouse rods.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Exploring the rate-limiting steps in visual phototransduction recovery by bottom-up kinetic modeling.Cell communication and signaling : CCS · 2013Article
- Speed, sensitivity, and stability of the light response in rod and cone photoreceptors: facts and models.Progress in retinal and eye research · 2012Review
- Spatiotemporal cGMP dynamics in living mouse rods.Biophysical journal · 2012Article
- Barriers to diffusion in dendrites and estimation of calcium spread following synaptic inputs.PLoS computational biology · 2011Article
- Dynamics of mouse rod phototransduction and its sensitivity to variation of key parameters.IET systems biology · 2010Article
- Metabolic constraints on the recovery of sensitivity after visual pigment bleaching in retinal rods.The Journal of general physiology · 2009Article
- Diffusion of the second messengers in the cytoplasm acts as a variability suppressor of the single photon response in vertebrate phototransduction.Biophysical journal · 2008Article
- Three-dimensional architecture of murine rod outer segments determined by cryoelectron tomography.The Journal of cell biology · 2007Article
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
Excitation signals spread along photoreceptor outer segments away from the site of photon capture because of longitudinal diffusion of cGMP, a cytoplasmic second messenger. The quantitative features of longitudinal diffusion reflect the anatomical structure of the outer segment, known to be profoundly different in rod and cone photoreceptors. To explore how structural differences affect cytoplasmic diffusion and to assess whether longitudinal diffusion may contribute to the difference in signal transduction between photoreceptor types, we investigated, both theoretically and experimentally, the longitudinal diffusion of small, hydrophilic molecules in outer segments. We developed a new theoretical analysis to explicitly compute the longitudinal diffusion constant, Dl, in terms of outer segment structure. Using time-resolved fluorescence imaging we measured Dl of Alexa488 and lucifer yellow in intact, single cones and validated the theoretical analysis. We used numerical simulations of the theoretical model to investigate cGMP diffusion in outer segments of various species. At a given time interval, cGMP spreads further in rod than in cone outer segments of the same dimensions. Across all species, the spatial spread of cGMP at the peak of the dim light photocurrent is 3-5 microm in rod outer segments, regardless of their absolute size. Similarly the cGMP spatial spread is 0.7-1 microm in cone outer segments, independently of their dimensions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.