ArticleBiophysical journal2004
Chlamydomonas sensory rhodopsins A and B: cellular content and role in photophobic responses.
Article in Biophysical journal, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
38 citing papers in PubMed, 95 citations in OpenAlex.
- Light-dependent processes in algae across scales: from cellular physiology to ecological organization.Plant physiology · 2026Review
- Article
- Blue-shifted ancyromonad channelrhodopsins for multiplex optogenetics.bioRxiv : the preprint server for biology · 2025Article
- Robust optogenetic inhibition with red-light-sensitive anion-conducting channelrhodopsins.eLife · 2024Article
- Gating and ion selectivity of Channelrhodopsins are critical for photo-activated orientation of Chlamydomonas as shown by in vivo point mutation.Nature communications · 2022Article
- Article
- Probing Channelrhodopsin Electrical Activity in Algal Cell Populations.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Photoprotective strategies in the motile cryptophyte alga Rhodomonas salina-role of non-photochemical quenching, ions, photoinhibition, and cell motility.Folia microbiologica · 2019Article
- Channelrhodopsin-1 Phosphorylation Changes with Phototactic Behavior and Responds to Physiological Stimuli inThe Plant cell · 2019Article
- Analysis and modeling of the inverted bioconvection inHeliyon · 2018Article
- Targeting of Photoreceptor Genes inThe Plant cell · 2017Article
- An Animal-Like Cryptochrome Controls thePlant physiology · 2017Article
- Microbial Rhodopsins: Diversity, Mechanisms, and Optogenetic Applications.Annual review of biochemistry · 2017Review
- A Plant Cryptochrome Controls Key Features of thePlant physiology · 2017Article
- Mechanism divergence in microbial rhodopsins.Biochimica et biophysica acta · 2014Review
- Role of a helix B lysine residue in the photoactive site in channelrhodopsins.Biophysical journal · 2014Article
- Microbial and animal rhodopsins: structures, functions, and molecular mechanisms.Chemical reviews · 2014Review
- Review
- Characterization of a highly efficient blue-shifted channelrhodopsin from the marine alga Platymonas subcordiformis.The Journal of biological chemistry · 2013Article
- Intramolecular proton transfer in channelrhodopsins.Biophysical journal · 2013Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Two retinylidene proteins, CSRA and CSRB, have recently been shown by photoelectrophysiological analysis of RNAi-transformants to mediate phototaxis signaling in Chlamydomonas reinhardtii. Here we report immunoblot detection of CSRA and CSRB apoproteins in C. reinhardtii cells enabling assessment of the cellular content of the receptors. We obtain 9 x 10(4) CSRA and 1.5 x 10(4) CSRB apoprotein molecules per cell in vegetative cells of the wild-type strain 495, a higher value than that for functional receptor cellular content estimated previously from photosensitivity measurements and retinal extraction yields. Exploiting our ability to control the CSRA/CSRB ratio by transformation with receptor gene-directed RNAi, we report analysis of the CSRA and CSRB roles in the photophobic response of the organism by action spectroscopy with automated cell tracking/motion analysis. The results show that CSRA and CSRB each mediate the photophobic swimming response, a second known retinal-dependent photomotility behavior in C. reinhardtii. Due to the different light saturation and spectral properties of the two receptors, CSRA is dominantly responsible for photophobic responses, which appear at high light intensity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.