ArticleBiophysical journal2004
Divalent cations increase lipid order in erythrocytes and susceptibility to secretory phospholipase A2.
Article in Biophysical journal, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 35 citations in OpenAlex.
- Differential interactions of essential and toxic metal ions with biologically relevant phosphatidic acid and phosphatidylserine membranes.Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine · 2024Article
- Lipid Structure Determines the Differential Impact of Single Metal Additions and Binary Mixtures of Manganese, Calcium and Magnesium on Membrane Fluidity and Liposome Size.International journal of molecular sciences · 2023Article
- The degree and position of phosphorylation determine the impact of toxic and trace metals on phosphoinositide containing model membranes.BBA advances · 2021Article
- Tetracycline hypersensitivity of an ezrA mutant links GalE and TseB (YpmB) to cell division.Frontiers in microbiology · 2015Article
- Voltage-dependent conformational changes in connexin channels.Biochimica et biophysica acta · 2012Review
- Biophysical effects of the natural product euplotin C on the Paramecium membrane.Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology · 2009Article
- The influence of membrane physical properties on microvesicle release in human erythrocytes.PMC biophysics · 2009Article
- Use of fluorescence to determine the effects of cholesterol on lipid behavior in sphingomyelin liposomes and erythrocyte membranes.Journal of lipid research · 2008Article
- Calcium triggered L alpha-H2 phase transition monitored by combined rapid mixing and time-resolved synchrotron SAXS.PloS one · 2008Article
- Effects of cholesterol on physical properties of human erythrocyte membranes: impact on susceptibility to hydrolysis by secretory phospholipase A2.Biophysical journal · 2008Article
- Use of steady-state laurdan fluorescence to detect changes in liquid ordered phases in human erythrocyte membranes.The Journal of membrane biology · 2006Article
- Domain-induced activation of human phospholipase A2 type IIA: local versus global lipid composition.Biophysical journal · 2006Article
- Mechanisms governing the level of susceptibility of erythrocyte membranes to secretory phospholipase A2.Biophysical journal · 2005Article
- Red blood cell membrane fluctuations and shape controlled by ATP-induced cytoskeletal defects.Biophysical journal · 2005Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Elevated concentrations of intracellular calcium in erythrocytes increase membrane order and susceptibility to secretory phospholipase A2. We hypothesize that calcium aids the formation of domains of ordered lipids within erythrocyte membranes by interacting directly with the inner leaflet of the cell membrane. The interface of these domains with regions of more fluid lipids may create an environment with weakened neighbor-neighbor interactions that would facilitate phospholipid migration into the active site of bound secretory phospholipase A2. This hypothesis was investigated by determining the effects of seven other divalent ions on erythrocyte membrane properties. Changes in membrane order were assessed with steady-state fluorescence spectroscopy and two-photon microscopy with an environment-sensitive probe, laurdan. Each ion increased apparent membrane order in model membranes and in erythrocytes when introduced with an ionophore, suggesting that direct binding to the inner face of the membrane accounts for the effects of calcium on membrane fluidity. Furthermore, the degree to which ions affected membrane properties correlated with the ionic radius and electronegativity of the ions. Lastly, erythrocytes became more susceptible to enzyme hydrolysis in the presence of elevated intracellular levels of nickel and manganese, but not magnesium. These differences appeared related to the ability of the ions to induce a transition in erythrocyte shape.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.