ArticleBiophysical journal2004
Temperature dependence of the surface topography in dimyristoylphosphatidylcholine/distearoylphosphatidylcholine multibilayers.
Article in Biophysical journal, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Role of the Surface in Conformational Changes in Lysozymes: Effect of a Gold Surface and a Lipid Membrane.International journal of molecular sciences · 2025Article
- Development, structure and mechanics of a syntheticNanoscale advances · 2021Article
- Biological physics by high-speed atomic force microscopy.Philosophical transactions. Series A, Mathematical, physical, and engineering sciences · 2020Review
- Thickness Mismatch of Coexisting Liquid Phases in Noncanonical Lipid Bilayers.The journal of physical chemistry. B · 2016Article
- Membrane restructuring by phospholipase A2 is regulated by the presence of lipid domains.Biophysical journal · 2011Article
- FCS diffusion laws in two-phase lipid membranes: determination of domain mean size by experiments and Monte Carlo simulations.Biophysical journal · 2011Article
- Investigation of SNARE-Mediated Membrane Fusion Mechanism Using Atomic Force Microscopy.Japanese journal of applied physics (2008) · 2009Article
- Characterizing the interactions between GPI-anchored alkaline phosphatases and membrane domains by AFM.Pflugers Archiv : European journal of physiology · 2008Review
- Atomic force microscope studies of the fusion of floating lipid bilayers.Biophysical journal · 2007Article
- The supramolecular architecture of junctional microdomains in native lens membranes.EMBO reports · 2007Article
- Coexisting stripe- and patch-shaped domains in giant unilamellar vesicles.Biochemistry · 2006Article
- Effect of temperature on the nanomechanics of lipid bilayers studied by force spectroscopy.Biophysical journal · 2005Article
- Coexistence of a two-states organization for a cell-penetrating peptide in lipid bilayer.Biophysical journal · 2005Article
- Lipid bilayers on polyacrylamide brushes for inclusion of membrane proteins.Langmuir : the ACS journal of surfaces and colloids · 2005Article
- Main phase transitions in supported lipid single-bilayer.Biophysical journal · 2005Article
- Biophysical interactions with model lipid membranes: applications in drug discovery and drug delivery.Molecular pharmaceuticsReview
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2 authors.
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Abstract
Simple lipid binary systems are intensively used to understand the formation of domains in biological membranes. The size of individual domains present in the gel/fluid coexistence region of single supported bilayers, determined by atomic force microscopy (AFM), generally exceeds by two to three orders of magnitude that estimated from multibilayers membranes by indirect spectroscopic methods. In this article, the topography of equimolar dimyristoylphosphatidylcholine/distearoylphosphatidylcholine (DMPC/DSPC) multibilayers, made of two superimposed bilayers supported on mica surface, was studied by AFM in a temperature range from room temperature to 45 degrees C. In the gel/fluid phase coexistence region the size of domains, between approximately 100 nm and a few micrometers, was of the same order for the first bilayer facing the mica and the top bilayer facing the buffer. The gel to fluid phase separation temperature of the first bilayer, however, could be increased by up to 8 degrees C, most likely as a function of the buffer layer thickness that separated it from the mica. Topography of the top bilayer revealed the presence of lipids in ripple phase up to 38-40 degrees C. Above this temperature, a pattern characteristic of the coexistence of fluid and gel domains was observed. These data show that difference in the size of lipid domains given by AFM and spectroscopy can hardly be attributed to the use of multibilayers models in spectroscopy experiments. They also provide a direct evidence for metastable ripple phase transformation into a gel/fluid phase separated structure upon heating.
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