ArticleBiophysical journal2004
Functional influence of the pore helix glutamate in the KcsA K+ channel.
Article in Biophysical journal, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
13 citing papers in PubMed.
- Inferring functional units in ion channel pores via relative entropy.European biophysics journal : EBJ · 2021Article
- Protonation state of E71 in KcsA and its role for channel collapse and inactivation.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Mechanism for selectivity-inactivation coupling in KcsA potassium channels.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Inactivation of the KcsA potassium channel explored with heterotetramers.The Journal of general physiology · 2010Article
- Conformational changes in the selectivity filter of the open-state KcsA channel: an energy minimization study.Biophysical journal · 2008Article
- Local and global structure of the monomeric subunit of the potassium channel KcsA probed by NMR.Biochimica et biophysica acta · 2007Article
- The protonation state of the Glu-71/Asp-80 residues in the KcsA potassium channel: a first-principles QM/MM molecular dynamics study.Biophysical journal · 2007Article
- Measurement of 15N relaxation in the detergent-solubilized tetrameric KcsA potassium channel.Journal of biomolecular NMR · 2006Article
- Structural and functional consequences of an amide-to-ester substitution in the selectivity filter of a potassium channel.Journal of the American Chemical Society · 2006Article
- In the yeast potassium channel, Tok1p, the external ring of aspartate residues modulates both gating and conductance.Pflugers Archiv : European journal of physiology · 2005Article
- Functional role and affinity of inorganic cations in stabilizing the tetrameric structure of the KcsA K+ channel.The Journal of general physiology · 2005Article
- A homology model of the pore region of HCN channels.Biophysical journal · 2005Article
- A conserved arginine near the filter of Kir1.1 controls Rb/K selectivity.Channels (Austin, Tex.)Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
Abstract
The E71 residue is buried near the selectivity filter in the KcsA K+ channel and forms a carboxyl-carboxylate bridge with D80. We have investigated the importance of E71 by examining neutralization mutants at this position using biochemical and electrophysiological methods. E71 mutations differentially destabilize the detergent-solubilized tetramer; among them, the E71V neutralization mutant has a relatively subtle effect. The E71V channel displays electrical activity when reconstituted into planar lipid bilayers. In single channel recordings, the mutant retains K+/Na+ selectivity, and its conductance in the outward direction is unaltered. Some conduction properties are changed: inward conductance is increased. Our results show that that the E71 side chain is not a primary determinant of ion selectivity or conduction in the wild-type channel, either directly or through the E71:D80 carboxyl-carboxylate bridge.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.